水产学报2017，Vol.41Issue(2)：221-229,9.DOI:10.11964/jfc.20160410354
加利福尼亚红刺参胚胎及幼体发育过程
Studies on embryonic and larval development of red giant sea cucumber (Parastichopus californicus)
摘要
Abstract
The red giant sea cucumber (Parastichopus californicus) is native to the west coast of North America,and is under quota fishery in the United State of America and Canada.As a result of global over-fishing of sea cucumbers and increasing market demand,it is in urgent need to develop artificial breeding ofP.californicus to promote the development of aquaculture.In order to develop artificial breeding ofP.californicus,the embryonic and larval development ofP.californicus was observed and recorded in the present study,and the size variations of fertilization eggs,embryos and larvae were measured under a microscope.The results showed that the gonad of P.californicus could be ripened by artificial diets,and the spawning of brood stock could be induced by desiccation method,in which the artificial fertilization rate exceeded 95％.The embryonic and larval development of P.californicus can be divided into the following stages:fertilization egg,cleavage,blastula,rotary blastula,gastrula,early aurieularia,mid aurieularia,late auricularia,doliolaria,pentactula,and juvenile.At the water temperature of 14 ℃C,the salinity of 30 and pH of 8,the polar body occurred in 10 to 20 minutes after fertilization,and the eggs started cleavage in 1 hour,and went into rotating blastula stage in 1 day,and went into gastrula in 2 days,and went into early auricularia in 4 days.It took a total of 15 days for fertilization eggs to develop into doliolaria stage,and 18 days to develop into juvenile stage.The auricularia P.californicus reached a maximum body length of 1125.0 μm.This is the first study on embryonic and larval development of P.californicus,which may provide scientific basis for the hatchery rearing,and artificial breeding of red giant sea cucumber.关键词
加利福尼亚红刺参/胚胎/幼体/发育过程/人工繁育Key words
Parastichopus californicus/embryo/larvae/development process/artificial breeding分类
生物科学引用本文复制引用
夏斌,任贻超,王永香,王文琪,郭恩棉,于丹..加利福尼亚红刺参胚胎及幼体发育过程[J].水产学报,2017,41(2):221-229,9.基金项目
山东省优秀中青年科学家科研奖励基金(BS2015HZ004) （BS2015HZ004）
青岛市自主创新计划(青年专项19-9-1-89-jch) （青年专项19-9-1-89-jch）
青岛农业大学博士基金(1113329)The Promotive Research Fund for Young and Middle-aged Scientists of Shandong Province (BS2015HZ004) （1113329）
Doctor Science Research Foundation of Qingdao City (15-9-1-89-jch) （15-9-1-89-jch）
Natural Science Foundation of Qingdao Agricultural University (1113329) （1113329）
