水产学报2017，Vol.41Issue(2)：311-318,8.DOI:10.11964/jfc.20160210297
红藻糖苷的提取及其对草鱼鱼糜抗冻性能的影响
Extraction of floridoside and the effect of floridoside on Ctenopharyngodon idella surimi during frozen storage
摘要
Abstract
To optimize the ethanol extraction conditions of floridoside and to investigate the effect of floridoside on the myofibrillar protein freezing denaturation of grass crap surimi during frozen storage.Ethanol concentration,extraction temperature,extraction duration and liquor to material ratio were optimized by response surface methods.The salt-solubility of myofibrillar protein,sulfhydryl content,and Ca2+-ATPase activity,etc in surimi after being frozen for 6 weeks were detected.Results showed that the optimal extracting condition for floridoside was 72.3％ ethanol with liquor to material ratio of 14:1 (mL/g),the extracting tempreture was 60 ℃C,and the extracting time should be 4 h.Under these optimal conditions,the highest extraction ratio was 3.48％.Salt-soluble protein,sulfhydryl group contents,Ca2+-ATPase activity were effectively enhanced,and the water loss was decreased by addition of floridoside in surimi.After 4 weeks frozen,salt-soluble protein and sulfhydryl group contents were enhanced by 30.62％ and 32.80％;Ca2+-ATPase activity in myofibrillar proteins was reduced by 37.51％ in 10％ floridoside treated group,while the water loss ratio was 133.07％ lower than that of the control group.This study indicates that floridoside can effectively prevent myofibrillar protein denaturation during frozen storage.关键词
草鱼/鱼糜/红藻糖苷/响应面/冷冻变性Key words
Ctenophyngodon idella/surimi/floridoside/response surface method/protein freezing denaturation分类
农业科技引用本文复制引用
高玉丽,李九零,严小军,杨锐,张春丹,陈海敏..红藻糖苷的提取及其对草鱼鱼糜抗冻性能的影响[J].水产学报,2017,41(2):311-318,8.基金项目
国家公益性行业(海洋)科研专项(201505033) （海洋）
宁波市科技攻关项目(2014C10044)National Public Welfare Industry Project Special Marine Scientific Research Funds (201505033) （2014C10044）
Ningbo Science and Technology Research Projects (2014C10044) （2014C10044）
