山东农业大学学报（自然科学版）2017，Vol.48Issue(1)：1-6,6.DOI:10.3969/j.issn.1000-2324.2017.01.001
Botryosphaeria dothidea突变体库的构建及分析
Construction and Evaluation of the Mutant Library of Botryosphaeria dothidea
摘要
Abstract
Botryosphaeria dothidea is an important pathogen of forest and fruit trees and it is widely distributed and causes serious damage. In this study, mutant library of B. dothidea was constructed via ATMT mediated transformation and 1053 transformants were obtained. Hygromycin B resistance genes were integrated into the genome of the B. dothidea, and mitotically stable after several subcultures were tested by PCR amplification and Southern blot. The strain B. dothidea sdau11-126 was used as control to analyze colonial morphology, growth rate and pathogenicity of 526 transformants. Eight stable and obvious mutants were obtained by screening. This study will lay a foundation for isolating, cloning and functional identification of the pathogenic genes.关键词
Botryosphaeriadothidea/农杆菌介导转化(ATMT)/突变体/筛选Key words
Botryosphaeria dothidea/Agrobacterium tumefaciens-mediated transformation(ATMT)/mutants/screening分类
农业科技引用本文复制引用
郑伟,贾晓曼,王玉珂,王怡霖,孙庚午,何邦令,刘会香..Botryosphaeria dothidea突变体库的构建及分析[J].山东农业大学学报（自然科学版）,2017,48(1):1-6,6.基金项目
国家重点研发计划(2016YFD0201100) （2016YFD0201100）
国家科技部基础平台项目中国树木溃疡病重要病原微生物多样性及在中国的生态地理分布和危害调查(2009FY210100) （2009FY210100）
