山东中医药大学学报2017，Vol.41Issue(2)：178-181,4.
高速逆流色谱法分离纯化黄芪中毛蕊异黄酮苷和芒柄花苷
Separation and Purification of Calycosin-7-glucoside and Ononin from Astragalus Membranceus by High-speed Counter-current Chromatography
摘要
Abstract
Objective:To establish the method of separating and purifying the calycosin-7-glucoside and ononin from astragalus membranceus by high-speed counter-current chromatography. Methods:The crude extract of astragalus membranceus was separated and purified by HSCCC with a two-phase solvent system com-posed of n-hexane-ethyl acetate-methanol-water ( 1:10:2:10 ) and ethyl acetate-ethanol-n-butyl alcohol-water (30:10:6:50) respectively. The lower phases were used as mobile phases with two flow rates of 2.5 mL·min-1 and 2.0 mL·min-1,while the apparatus rotated at 900 r·min-1 and the wavelength were detected at 254 nm. The structures of the target compounds were identified by HPLC. Results:From 300 mg crude extract of as tragalus membranceus,two compounds with calycosin-7-glucoside (20 mg) and ononin (25 mg) were obtained. The purities of these two compounds determined by HPLC were both over 95%. Conclusion:High-speed counter-current chromatography is an efficient method for separating and purifying the calycosin-7-glucoside and ononin from astragalus membranceus.关键词
高速逆流色谱/黄芪/毛蕊异黄酮苷/芒柄花苷Key words
high-speed counter-current chromatography/astragalus membranceus/calycosin-7-glucoside/ononin分类
医药卫生引用本文复制引用
赵竞业,容蓉,巩丽丽,陈迩东,蒋海强..高速逆流色谱法分离纯化黄芪中毛蕊异黄酮苷和芒柄花苷[J].山东中医药大学学报,2017,41(2):178-181,4.基金项目
山东中医药大学创新训练项目(编号:2014408) （编号:2014408）
