山东医药2016，Vol.56Issue(48)：1-4,4.DOI:10.3969/j.issn.1002-266X.2016.48.001
对顺铂耐药的骨肉瘤细胞干细胞特性变化及其与Notch信号通路的关系
Changes of stem cell properties in cisplatin-resistant osteosarcoma cells and its relationship with Notch signaling pathway
摘要
Abstract
Objective To investigate the characteristics of cisplatin-resistant osteosarcoma stem cells and its relationship with Notch signaling pathway.Methods The cisplatin-resistant human osteosarcoma 143B-TK cells in the logarithmic growth phase (observation group) were selected,and 143B-TK cells without the treatment of cisplatin were taken as the control group.Real-time quantitative PCR was used to detect the expression of the stem cells-related genes (TERT,Sox2,Oct4 and NANOG),and the Notch signaling pathway-related genes (HeyL,Hey2,Hey1,Hes5,and Hes1) in the two groups.Cell forming ability was detected by the formation of sphere number in the two groups with or without adding DAPT.Results No significant difference was found in the relative expression of NANOG and Hey2 between the two groups (all P ＞0.05).The relative expression of TERT,Sox2,Oct4,HeyL,Hey1,Hes5,and Hes1 in the observation group was higher than that in the control group (P ＜0.05 or P ＜0.01).The sphere formation number in the observation group without adding DAPT was more than that in the observation group with adding DAPT and that in the control group without adding DAPT (allP＜0.01).Conclusion The stem cell properties ofcisplatin-resistant osteosarcomacells enhance,and the activation of Notch signaling pathway may be main reason for the enhancement of stem cell properties and drug resistance.关键词
骨肉瘤/顺铂耐药/肿瘤干细胞/Notch信号通路Key words
osteosarcoma/chemotherapy resistance/cancer stem cells/Notch signaling pathway分类
医药卫生引用本文复制引用
李提,方硕,余铃,杨俭,郭卫春..对顺铂耐药的骨肉瘤细胞干细胞特性变化及其与Notch信号通路的关系[J].山东医药,2016,56(48):1-4,4.基金项目
国家自然科学基金资助项目(81341078). （81341078）
