沈阳大学学报（自然科学版）2017，Vol.29Issue(1)：14-20,7.
17β雌二醇刺激农田土壤微生物群落结构变化PCR-DGGE指纹分析
Analysis of Effect of Estradiol on Microbial Community Structure in Agricultural Soil Using PCR-DGGE Fingerprinting
摘要
Abstract
The polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) method is used to analyze the variation of the microbial community structure in estradiol-contaminated agricultural soil samples with different mass fraction and time of exposure.The results demonstrate that the growth of some bacterial species in the estradiol-contaminated agricultural soil samples is inhibited during the exposure.The number of some bacteria decreases with the increase of estradiol concentration or time of exposure, while other bacteria are enriched under the effect of estradiol, and their DGGE bands change from undertones to deep colors.The microbial community structure, however, show a wide variation in the estradiol contaminated agricultural soil samples, especially in the agricultural soil samples treated with estradiol concentration of 200ng/kg.The diversity indices of microbial community with concentration of 200ng/kg are greater than those of another two groups.Diversity indices show that the contaminated group of 100ng/kg is decreased with the increase of exposure time, while the contaminated groups of 200ng/kg and 300ng/kg have a similar moderate growth trend over time.It is found that suitable mass fraction of estradiol could increase the diversity of the microbial community in the agricultural soil.关键词
17β雌二醇/农田土壤/微生物群落结构/PCR-DGGEKey words
17β-estradiol/agricultural soil/microbial community structure/PCR-DGGE分类
资源环境引用本文复制引用
田琳,张珣..17β雌二醇刺激农田土壤微生物群落结构变化PCR-DGGE指纹分析[J].沈阳大学学报（自然科学版）,2017,29(1):14-20,7.基金项目
国家自然科学基金资助项目(41472237) （41472237）
辽宁省高等学校优秀人才支持计划项目(LJQ2015077) （LJQ2015077）
辽宁省科学技术计划资助项目(201602530). （201602530）
