沈阳大学学报（自然科学版）2017，Vol.29Issue(1)：21-25,5.
土霉素降解菌的筛选及其降解条件优化
Screening and Optimization of Degradation Condition of Oxytetracycline Degrading Bacteria
摘要
Abstract
An oxytetracycline-degrading strain from the soil of poultry breeding farms in Huludao City was isolated by enrichment culture method and its degradation conditions were optimized.The strain named YH1 was identified based on the morphology observation;the colony morphology was gray, irregular margin, and the center was raised like a straw hat.By 16S rDNA gene sequence, the preliminary identification suggested that the strain belonged to Brevundimonas sp.When the temperature was 30℃, pH was 6.0, and mass concentration of oxytetracycline was 300mg/L, the degradation rate of the bacterial strain was up to 70.34%.Adding different carbon or nitrogen sources and metal ions had an impact on the degradation of strains;when 2g/L soluble starch and maltose were added, the degradation rate of the strain was increased;when 1g/L of Fe3+ and Cu2+ was added, the degradation rate of the strain was increased;while Cd2+ and Mn2+ could inhibit the degradation rate.关键词
土霉素/微生物降解/条件优化/16SrDNAKey words
oxytetracycline/microbial degradation/condition optimization/16S rDNA分类
资源环境引用本文复制引用
于浩,李晔,程全国..土霉素降解菌的筛选及其降解条件优化[J].沈阳大学学报（自然科学版）,2017,29(1):21-25,5.基金项目
国家自然科学基金资助项目(31470552) （31470552）
辽宁省教育厅资助项目(L2012405). （L2012405）
