实验动物与比较医学2017，Vol.37Issue(1)：1-5,5.DOI:10.3969/j.issn.1674-5817.2017.01.001
FGF21siRNA对STZ诱导的1型糖尿病小鼠心脏功能的影响
Reduction of Cardiac Function Induced by FGF21siRNA in Type 1 Diabetic Mice
摘要
Abstract
Objective To investigate the effects of Fibroblast growth factor 21 (FGF21) siRNA on cardiac function in type 1 diabetic (T1DM) mice and explore the mechanisms of actions.Methods Sixteen C57BL/6J mice were randomly divided into,control group and FGF21siRNA control group,sixteen T1DM model mice were divided into T1DM group and T1DM + FGF21siRNA group,type 1 diabetes was induced by STZ.Cardiac function was analyzed by echocardiography,morphological changes of cardiomyocytes were observed by HE staining,collagen fiber was observed by Masson staining,the expression of α-myocin heavy chain (α-MHC),β-myocin heavy chain (β-MHC),atrial natriuretic peptide (ANP),type Ⅰ collagen (Col Ⅰ) and type Ⅲ collagen (Col Ⅲ) of myocardium were detected by Real-time PCR.Results The expression of α-MHC,β-MHC,ANP,Col Ⅰ and Col Ⅲ in myocardium of T1DM mice treated with FGF21siRNA were increased compared to those of untreated ones.Cardiac function,cardiomyocyte hypertrophy and collagen fiber proliferation of treated mice were worse than those of untreated ones.Conclusion FGF21siRNA may contribute to cardiac malfunction T1DM mice,which may associate with the development of myocardial hypertrophy,myocardial fibrosis.关键词
成纤维细胞生长因子21(FGF 21) siRNA/1型糖尿病(T1DM)/糖尿病心肌病/心肌肥大/心肌纤维化Key words
FGF21siRNA/Type 1 diabetic (T1DM)/Diabetic cardiomyopathy/Myocardial hypertrophy/Myocardial fibrosis分类
生物科学引用本文复制引用
陈翠,孟哲颖,郑元义,胡兵,申锷..FGF21siRNA对STZ诱导的1型糖尿病小鼠心脏功能的影响[J].实验动物与比较医学,2017,37(1):1-5,5.基金项目
国家自然科学基金面上项目(NO.81270208) （NO.81270208）
