实验动物与比较医学2017，Vol.37Issue(1)：6-10,5.DOI:10.3969/j.issn.1674-5817.2017.01.002
ND3在自发性糖尿病长爪沙鼠5种组织中的表达
Expression of ND3 in 5 Tissues of Hereditary Diabetic Mongolian Gerbils
摘要
Abstract
Objective To investigate the mRNA and protein expression level of NADH dehydrogenase 3(ND3) gene in 5 different tissues of diabetic gerbils,to understand the location and target tissue of ND3 in diabetic gerbil model.Methods Six gerbils for control and diabetic groups were selected respectively.The kidney,liver,skeletal muscle,brain and heart from each group were collected.The mRNA and protein expression of ND3 were evaluated by Real time-PCR and Western blotting in both groups.Results The mRNA expression of ND3 in diabetic group was extremely significantly increased in kidney,and significantly decreased in liver and skeletal muscle compared with those of control animal.While it just showed decreasing tendency in brain and increasing tendency in heart tissue.The detection of protein expression showed that the protein level was consistent with mRNA expression in all tissues except heart.Conclusions The kidney may be the target tissue of ND3 in diabetic gerbil.In addition,ND3 also exhibits effect in other 4 tissues of diabetic gerbil.关键词
长爪沙鼠/2型糖尿病/ND3基因/实时定量PCR/Western blottingKey words
Mongolian gerbils/Type 2 diabetes/ND3/Real-time PCR/Western blotting分类
生物科学引用本文复制引用
李银银,郭萌,李长龙,陈振文,杜小燕,龚菁菁,吴绍亮,李小红,王存龙,霍学云,路静,吕建祎,刘欣..ND3在自发性糖尿病长爪沙鼠5种组织中的表达[J].实验动物与比较医学,2017,37(1):6-10,5.基金项目
国家科技支撑计划(No.2015BAI09B01) （No.2015BAI09B01）
国家自然科学基金(Nos.31272393,31572348) （Nos.31272393,31572348）
北京自然基金(No.7141002) （No.7141002）
