实验动物与比较医学2017，Vol.37Issue(1)：20-24,5.DOI:10.3969/j.issn.1674-5817.2017.01.005
非酒精性脂肪性肝纤维化小鼠模型的建立及炎症因子的表达
Establishment of Nonalcoholic Fatty Liver Fibrosis Model and Expression of Inflammatory Factors in Mice
摘要
Abstract
Objective To establish the non-alcoholic fatty liver fibrosis model in mice and observe the pathological changes and the expression of inflammatory factors.Methods Twenty male C57BL/6J mice were randomly divided into control group and model group.The mice in control group were fed with methionine and choline-supplement(MCS) diet,and the model group were fed with methionine choline deficiency (MCD) diet.The mice were weighed and sacrificed at after 8 weeks feeding.The histopathological changes in liver were observed.The level of serum aspartate aminotransferase (AST),alanine aminotransferase (ALT),cholesterol (TC),triglyceride (TG),tumor necrosis factor-α (TNF-α) and interleukin (IL-6) were detected.Results The body weight of mice in the model group decreased significantly at the end of the study.Severe hepatic steatosis,liver cell edema,visible focal necrosis and focal lymphocytic infiltration,interface hepatitis,periportal enlargement,hyperplasia of fibrous tissue were observed.The scores of liver inflammation activity and fibrosis score in model group were significantly higher than those in control group (P＜0.01).The level of serum ALT、AST、TNF-α、IL-6 were significantly higher in the model group than that of control group (P＜0.01).Conclusion After feeding mice with MCD diet feeding for 8 weeks,non-alcoholic fatty liver fibrosis model of mice was successfully induced.The modeling method was simple,rapid,and with has high survival rate.It is suitable for the pathogenesis and drug intervention study of nonalcoholic fatty liver fibrosis.关键词
非酒精性脂肪性肝纤维化/蛋氨酸-胆碱缺乏(MCD)/C57BL/6J小鼠/炎症因子Key words
Non-alcoholic fatty liver Fibrosis/Methionine choline deficiency(MCD)/C57BL/6J mice/Inflammatory factors分类
生物科学引用本文复制引用
杨华,赵亚娟,欧强..非酒精性脂肪性肝纤维化小鼠模型的建立及炎症因子的表达[J].实验动物与比较医学,2017,37(1):20-24,5.基金项目
上海市第八人民医院人才引进科研基金(BY201406) （BY201406）
