实验动物与比较医学2017，Vol.37Issue(1)：25-31,7.DOI:10.3969/j.issn.1674-5817.2017.01.006
上海地区常用近交系小鼠品系的单核苷酸多态性分型研究
The Single Nucleotide Polymorphism Genotyping of Inbred Mice from Shanghai
摘要
Abstract
Objective To analyze the single nucleotide polymorphism (SNP) genotyping of inbred mice from Shanghai.Methods 44 SNP loci on 21 chromosomes of 10 inbred mice strains from 2 laboratory animal companies of Shanghai were detected by the PCR-LDR genotyping panels,and the other 2 inbred mice strains were selected as the double blind samples for verification.And discrepancy locus were verified by sequencing.Results The SNP genotyping results of all the samples from the same inbred mice strains were homomorphism,but 6 inbred mice strains from different populations (BALB/c,FVB,DBA/2 and C3H/He) were polymorphism with 7 and 2 discrepancy locus respectively.The sequencing results to verify the discrepancy locus were the same as the SNP genotyping results.Conclusions The SNP genotyping was very stable and consistent in inbred mice strains from the same population in Shanghai,and the SNP genotyping of the same inbred mice strains from different populations was polymorphism.关键词
近交系小鼠/遗传监测/单核苷酸多态性(SNP)分型Key words
Inbred mice/Genetic monitoring/Single nucleotide polymorphism (SNP) genotyping分类
生物科学引用本文复制引用
韩琳,谢建云,杨斐,胡樱,田立立,鲍世民..上海地区常用近交系小鼠品系的单核苷酸多态性分型研究[J].实验动物与比较医学,2017,37(1):25-31,7.基金项目
上海市科学技术委员会科研计划项目(14140900800) （14140900800）
