实验动物与比较医学2017，Vol.37Issue(1)：36-39,4.DOI:10.3969/j.issn.1674-5817.2017.01.008
不同因素对环磷酰胺诱导的免疫抑制小鼠模型效果的影响
Effect of Different Factors on Cyclophosphamide Induced Immunosuppression Mice Model
宋莹 1郭雅娟 2黄铭倩 1梁金强 1黄芝瑛2
作者信息
- 1. 中山大学实验动物中心,广州510006
- 2. 中山大学药学院,广州510006
- 折叠
摘要
Abstract
Objective To investigate the different dosages of cyclophosphamide (CY) and endpointdetection on the effects of immunosuppression model.Methods BALB/c mice were divided into 4 groups randomly:negtive control group (A),CY 40 mg/kg group (B),CY 60 mg/kg group (C),which were all tested at 5 days after the last administration,and CY 60 mg/kg group-2 (D) was tested at 3 days after.The animal's body weight was measured every day,hematological parameters and immune organ index were detected when the experiments finished,as well as immune function was mediated by T or B lymphocytes testing.Results The different doses of CY and endpoint-detection have impact on the immunosurpression effect.60 mg/kg CY has stronger immunosurpression effect when tested at 5 days after the last administration.The alteration of immune indicators were more obervious when tested at 3 days after the last administration compared with 5 days at the dosage of 60 mg/kg CY.Conclusion Dosages of CY and endpoint-detection have impact on immunosuppressed mice model.60 mg/kg CY is more effective than 40 mg/kg CY,and the results are better when 3 days after the last administration is chosen as the endpoint-detection.关键词
环磷酰胺(CY)/检测终点/免疫抑制/小鼠模型Key words
Cyclophosphamide (CY)/Endpoint-detection/Immunosuppression/Mice model分类
生物科学引用本文复制引用
宋莹,郭雅娟,黄铭倩,梁金强,黄芝瑛..不同因素对环磷酰胺诱导的免疫抑制小鼠模型效果的影响[J].实验动物与比较医学,2017,37(1):36-39,4.
