同济大学学报（医学版）2017，Vol.38Issue(1)：7-11,17,6.DOI:10.16118/j.1008-0392.2017.01.002
脯氨酰羟化酶抑制剂对PC12细胞SDF-1α表达的影响
Effects of prolyl hydroxylase domain inhibitors on SDF-1α expression in PC12 cells
摘要
Abstract
Objective To investigate the effects and underlying mechanisms of prolyl hydroxylase domain (PHD) inhibitors on the expressions of SDF-1α in PC12 cells.Methods Cultured rat pheochromocytoma PC12 cells were treated with different concentrations of PHD inhibitors cobalt chloride(CoCl2),desferrioxamine (DFO) or dimethyloxalylglycine (DMOG),respectively.Cell viability was determined with cell counting kit-8 (CCK-8);the expression of SDF-1α mRNA was detected with quantitative real time PCR;the expression of HIF-1α and SDF-1α protein was measured with Western blotting and ELISA assays,respectively.Results CCK-8 showed that low concentration of CoCl2 promoted cellular growth;however,DFO,DMOG and higher concentration of CoCl2 inhibited cell growth with concentration increasing.The mRNA level of SDF-1 α was down regulated in a concentration dependent manner by DFO,CoCl2 and DMOG.Western blotting showed that HIF-1 αlevels in PC12 cells treated with CoCl2,DFO or DMOG were higher than that in control group.ELISA analysis showed that SDF-1 α expression was significantly lower in PC12 cells treated with DFO,CoCl2 or DMOG.Conclusion Prolyl hydroxylase domain (PHD) inhibitors up-regulate the level of HIF-1 α and down-regulate the level of SDF-1α in rat pheochromocytoma PC12 cells.关键词
脯氨酰羟化酶抑制剂/低氧诱导因子-1α/基质细胞衍生因子-1α/去铁胺/氯化钴/二甲基乙二酰基甘氨酸Key words
prolyl hydroxylase domain inhibitors/HIF-1 α/SDF-1 α/DFO/CoCl2/DMOG分类
医药卫生引用本文复制引用
吴滢,王郁,赵延欣..脯氨酰羟化酶抑制剂对PC12细胞SDF-1α表达的影响[J].同济大学学报（医学版）,2017,38(1):7-11,17,6.基金项目
国家自然科学基金面上项目(81571033) （81571033）
上海市科委西医类引导项目(134119a6900) （134119a6900）
上海市教委重点资助项目(14ZZ044) （14ZZ044）
