同济大学学报（医学版）2017，Vol.38Issue(1)：30-35,6.DOI:10.16118/j.1008-0392.2017.01.006
AGEs-RAGE相互作用促进巨噬细胞摄取胆固醇的研究
AGEs-RAGE interaction increases the cholesterol uptake in macrophages
摘要
Abstract
Objective To investigate the effect of AGEs-RAGE axis on the cholesterol uptake of macrophage.Methods Human THP-1 monocytes were treated with PMA (100 ng/ml) for 48 h,and then induced with oxLDL (100 μg/ml) for differentiating to macrophage foam cells.Cells were pretreated by AGEs (300 or 600 μg/ml) for 2 h,and pre-stimulated with antibody for RAGE (10 μg/rnl).The oil red O staining and measurement of cholesterol ester were used for evaluating the accumulation of lipid in macrophages.RT-PCR and Western blotting analysis were used to test the mRNA and protein expression of RAGE,SRA2,CD36.The fluorescence intensity was tested for the ability of cholesterol uptake in macrophages.Results After induction of AGEs (600 μg/ml),the content of lipid in macrophage was increased,and the expression of RAGE,SRA2,CD36 were increased.The ability of macrophages for cholesterol uptake was enhanced.For blocking up the AGEs-RAGE axis by antibody to RAGE,all the changes were reversed.Conclusion AGEs-RAGE axis can promote cholesterol uptake in macrophages,which were transformed to foam cell.关键词
糖基化终末产物/糖基化终末产物受体/巨噬细胞/胆固醇/清道夫受体A2Key words
advanced glycation end products/receptor of advanced glycation end products/macrophage/cholesterol/scavenger receptors A2分类
医药卫生引用本文复制引用
徐雷,王一茹,李沛城,冯波..AGEs-RAGE相互作用促进巨噬细胞摄取胆固醇的研究[J].同济大学学报（医学版）,2017,38(1):30-35,6.基金项目
国家自然科学基金(81300699) （81300699）
上海市卫生与计划生育委员会青年科研项目(20124Y106) （20124Y106）
上海市浦东新区优秀青年医学人才计划(PWRq2013-04) （PWRq2013-04）
