同济大学学报（医学版）2017，Vol.38Issue(1)：36-39,43,5.DOI:10.16118/j.1008-0392.2017.01.007
氢气饱和生理盐水对内毒素诱导的小鼠急性肺损伤的保护作用
Protective effect of hydrogen-saturated saline on lipopolysaccharide-induced acute lung injury in mice
摘要
Abstract
Objective To evaluate the protective effect of hydrogen-rich saline (HRS) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.Methods Ninety male BABL/c mice were randomly divided into Sham group,ALI group and ALI+ HRS group with 30 mice in each group.Acute lung injury model was induced by intraperitoneal injection of LPS in ALI and ALI+ HRS groups.Intraperitoneal injection of hydrogen-rich saline was given to mice in ALI + HRS group 1 h after injection of LPS.Twelve mice in each group were selected and observed for the 7 d-survival rate.Three mice in each group were sacrificed at the 3rd,5th and 7th day after LPS injection,respectively.Pathological changes of the lungs were observed by H-E staining.The mRNA and protein expression of iNOS and Arg-1 in lung tissue was detected by qPCR and immunofluorescence staining,respectively.Results Compared with Sham group,the survival rate of ALI group was decreased;compared with ALI group,the survival rate of ALI+ HRS group was significantly increased.H-E staining showed that the inflammatory cell infiltration in lung tissue of ALI + HRS group was significantly decreased compared with ALI group.qPCR results showed that the expression of iNOS in ALI+ HRS group decreased,and the expression of Arg-1 increased compared with ALI group.The results of immunofluorescence staining were consistent with those of qPCR.Conclusion Intraperitoneal injection of saturated hydrogen saline can inhibit the expression of proinflammatory factor iNOS and promote the expression of inflammatory factor Arg-1,which can reduce endotoxin induced lung injury in mice.关键词
急性肺损伤/氢气饱和生理盐水/脂多糖/诱导型一氧化氮合酶/精氨酸酶1Key words
acute lung injury/hydrogen-saturated saline/lipopolysaccharide/nitric oxide synthase/Arg-1分类
医药卫生引用本文复制引用
盛洁莹,谢旺,厉旭光,张洁,唐瑾,郭忠良..氢气饱和生理盐水对内毒素诱导的小鼠急性肺损伤的保护作用[J].同济大学学报（医学版）,2017,38(1):36-39,43,5.基金项目
国家自然科学基金(81370174) （81370174）
