色谱2017，Vol.35Issue(3)：264-268,5.DOI:10.3724/SP.J.1123.2016.10010
毛细管离子色谱-串联质谱法测定热纤梭杆菌发酵液中的低相对分子质量有机酸
Capillary ion chromatography-mass spectrometry for determination of low relative molecular mass organic acids in culture media of Clostridium thermocellum
摘要
Abstract
A capillary ion chromatography-mass spectrometry method was proposed to determine low relative molecular mass organic acids in culture media of Clostridium thermocellum.The conditions of analysis were optimized and eight organic acids were detected simultaneously.The target compounds were separated on an IonSwift MAX-100 capillary column with potassium hydroxide solution as mobile phase by gradient elution, and detected under selected ion monitoring (SIM) mode.The mass spectrometry conditions were: spray voltage 3.0 kV, sheath gas 2 000 kPa, capillary temperature 275 ℃.The relative standard deviations ranged from 1.45% to 5.99%.The coefficients of determination ranged from 0.9696 to 0.9986 and the mean recoveries were 89.0%-110.0%.The limits of detection of the eight organic acids were 0.01-0.50mg/L.This method was used to detect low relative molecular mass organic acids in culture media of Clostridium thermocellum with high sensitivity and good reproducibility.关键词
毛细管离子色谱/质谱/有机酸/热纤梭杆菌Key words
capillary ion chromatography/mass spectrometry (MS)/organic acids/Clostridium thermocellum分类
化学化工引用本文复制引用
赵海杰,梁文辉,李震,史庆昊,建方方,法芸,刘会洲..毛细管离子色谱-串联质谱法测定热纤梭杆菌发酵液中的低相对分子质量有机酸[J].色谱,2017,35(3):264-268,5.基金项目
国家自然科学基金项目(21405166) （21405166）
科技部重大专项(2012YQ090229).National Natural Science Foundation of China (No.21405166) （2012YQ090229）
Major Projects of the Ministry of Science and Technology (No.2012YQ090229). （No.2012YQ090229）
