生物骨科材料与临床研究2017，Vol.14Issue(1)：10-15,后插2-后插3,8.DOI:10.3969/j.issn.1672-5972.2017.01.003
三维打印双相磁性纳米复合支架材料的性能测定
Determination of the three-dimensional magnetic properties of duplex printing nanocomposite scaffold
摘要
Abstract
Objective Study prepared a novel two-phase magnetic nanocomposite scaffolds (PLGA/Col-I-PLGA/n-HA/ Fe2O3),through various biological performance testing,evaluation and to explore the feasibility of bone tissue engineering scaffolds.Methods Preparation of two-phase magnetic nanocomposite scaffolds (PLGA/Col-I-PLGA/n-HA/Fe2O3) by cryogenic rapidprototyping method,using electronic detection machine scaffold flexural,compressive strength,elastic modulus to evaluate the mechanical properties by electron microscopy ultrastructure scaffold;in the medium (ethanol) immersion assay scaffold porosity between the scaffold and bone marrow mesenchymal stem cells co-cultured detect its biocompatibility.Results Duplex magnetic nanocomposite scaffold mechanical test results show it has good mechanical properties,electron microscopy showed uniform pore size distribution of the upper and lower layers,the upper layer of cartilage with smaller pore size,good fusion between successive phase,pore size and porosity of the test results showed cartilage aperture layer scaffold to 189um,a porosity of 86.5％.Aperture bone scaffold layer is 364um,a porosity of 77.1％,in line with the double scaffold design requirements.Duplex between magnetic nano composite scaffold co-cultured with bone marrow mesenchymal stem cells,showed that bone marrow mesenchymal stem cell proliferation effect is very good,better able to promote the differentiation of cells for the purpose described biphasic magnetic nanocomposite scaffolds have good biocompatibility.Conclusion Duplex magnetic nanocomposite scaffolds (PLGA/Col-I-PLGA/n-HA/Fe2O3) has good mechanical properties and biocompatibility,pore size and pore growth rate of cell adhesion requirements,and normaljoint subchondral bone and cartilage physiological structure closer,is expected to be able to better repair osteoarthritis and other diseases caused by trauma or cartilage and subchondral bone damage under.关键词
低温快速成型/3D打印/双相磁性纳米Key words
Low temperature rapid prototyping/3D printing/Duplex magnetic nano分类
医药卫生引用本文复制引用
黄江鸿,陈洁琳,刘启颂,贾兆锋,王大平,熊建义,丁文斌,王大明,刘威,尤微,朱伟民,段莉..三维打印双相磁性纳米复合支架材料的性能测定[J].生物骨科材料与临床研究,2017,14(1):10-15,后插2-后插3,8.基金项目
深圳市科技研发资金项目(编号:CXZZ20140813160132596) （编号:CXZZ20140813160132596）
