生物骨科材料与临床研究2017，Vol.14Issue(1)：16-19,后插4-后插5,6.DOI:10.3969/j.issn.1672-5972.2017.01.004
尼古丁在人椎间盘髓核细胞退变中的作用
The effect of nicotine on human nucleus pulposus cells
摘要
Abstract
Objective To evaluate the effect of nicotine on the degeneration of human nucleus pulposus cells.Methods 5 patients undergoing lumbar discectomy surgeries in our department were randomly included.The nucleus pulposus tissues were cultured in well plate and the concentrations of nicotine were 0,1,33.3,100,200,500ng/mL.After 1,2,3,7,10 days,RNA of cells were extracted using Trizol.The mRNA expression ofAQP1,AQP3,collagen Ⅱ and Aggrecan were valued by rtPCR.Data analysis was done by SPSS19.0.Results With an increase in nicotine concentration and culture time,the morphology of nucleus pulposus changed significantly,weakening of the adherent ability,showing decrease of cytoplasm,atrophy of cell nucleus and increase ofapotosis.The mRNA expression ofAQP 1 and AQP3 also decreased and so did collagen Ⅱ and aggrecan.Conclusion Nicotine can decrease the mRNA expression of AQP 1and AQP3 of nucleus pulposus,thus,causing a decrease in the expression of type Ⅱ collagen and aggrecanto accelerate disc degeneration.This effect seems to be time and dose dependent.关键词
尼古丁/椎间盘/髓核细胞/退变/水通道蛋白Key words
Nicotine/Intervertebral disc/Nucleus pulposus cell/Degeneration/Aquaporins分类
医药卫生引用本文复制引用
黄宇峰,雍之瑶,刘晓明,李浩曦,王善金,巴兆玉,沈彬,赵卫东,吴德升..尼古丁在人椎间盘髓核细胞退变中的作用[J].生物骨科材料与临床研究,2017,14(1):16-19,后插4-后插5,6.基金项目
国家自然科学基金面上项目(81672199) （81672199）
上海市教委产学研项目课题(15cxy50) （15cxy50）
2013年上海市东方医院朝阳人才计划(DFZY-8) （DFZY-8）
浦东新区卫生系统重点学科建设资助(PWzx2014-02) （PWzx2014-02）
