摘要
Abstract
Escherichia coli expression system has become the first choice of expression of exogenous protein,the expression system for recombinant protein presents many advantages,and however,sometimes expressed exogenous protein is easily attacked by host protease or cannot form insoluble inclusion bodies because of incorrect folding,thus by which the expression is limited.This paper summarizes some optimization strategies used in the process of the expression of exogenous proteins in E.coli,mainly including the expression of rare codons,application of fusion tags,change of E.coli strains for expression,and reducing the formation of inclusion bodies,a single protein production system,auto-induction,fed-batch cultivation,high cell-density culture,etc.,which aims at exploring the optimal strategy for the expression of exogenous protein.关键词
大肠杆菌/外源蛋白/流加培养/高密度培养Key words
Escherichia coli/exogenous protein/fed-batch cultivation/high-cell-density cultivation
