生殖医学杂志2017，Vol.26Issue(2)：163-167,5.DOI:10.3969/j.issn.1004-3845.2017.02.012
Synaptotagmin1影响小鼠卵母细胞减数分裂和皮质反应的研究
Synaptotagmin 1 affects meiosis and cortical reaction in mouse oocytes
摘要
Abstract
Objective:To identify the effect of Synaptotagmin 1 (Syt1) on meiosis and cortical reaction in mouse oocyte.Methods:Syt1 expression was determined by immunoblotting analysis.The localization of Syt1 in oocytes and the effect on meiosis processes were analyzed by immunofluorescence.Cortical reaction and the dynamics of microtubule and chromosome were filmed with Perkin Elmer precisely Ulta VIEW VOX Confocal Imaging System.Results:Syt1 expression was localized at the same position as cortical granules(CGs) in mouse oocyte.Down-regulation of Syt1 expression interrupted the meiotic process in mouse oocyte.The results of Perkin Elmer precisely Ulta VIEW VOX Confocal Imaging System confirmed that down-regulation of Syt1 affected MI and MII conversion,the first polar body release and further cortical response.Conclusions:Syt1 is involved in mouse oocyte meiotic maturation process and cortical reaction.关键词
Synaptotagmin1/减数分裂/皮质反应/卵母细胞Key words
Synaptotagmin1/Meiosis/Cortical reaction/Oocyte引用本文复制引用
朱秀兰,易艳红,唐婷,张春晖,刘风华..Synaptotagmin1影响小鼠卵母细胞减数分裂和皮质反应的研究[J].生殖医学杂志,2017,26(2):163-167,5.基金项目
国家自然科学基金(2013-81300479)&深圳市科创委面上项目(JCY20120616144719076) （2013-81300479）
