天津科技大学学报2017，Vol.32Issue(1)：1-12,12.DOI:10.13364/j.issn.1672-6510.20160288
莱茵衣藻作为绿色细胞工厂生产高附加值 重组蛋白的研究进展
Recent Progress in Developing Chlamydomonas reinhardtii as a Green Cell Factory for Producing High-value Recombinant Proteins
摘要
Abstract
Recombinant proteins are widely used in fields like medical treatment,cultivation and food industry.The unicel-lular green algae C.reinhardtii has since long time ago attracted great attention for its use as a green cell factory to produce recombinant proteins.Compared with traditional cell factories,this photosynthetic eukaryotic microbe has many advantages in the production of high-value recombinant proteins,due to its abilities of accurately folding and assemblying complex proteins,short incubation time,high bio-safety level,low production costs and so on.At present,C.reinhardtii chloroplast genome and nuclear genome have been successfully used to express a variety of recombinant proteins,such as subunit protein vaccines,single chain antibodies,cytokines,hormones and cultivated enzymes for industrial use,etc.These achievements have greatly verified the usefulness of C.reinhardtii as a green cell factory.In this article,the potentials of C.reinhardtii as a green cell factory to produce high-value recombinant proteins are summarized,successful cases for its usefulness as a green cell factory are presented and the shortcomings as an efficient green cell factory is discussed.关键词
莱茵衣藻/绿色细胞工厂/重组蛋白Key words
Chlamydomonas reinhardtii/green cell factory/recombinant protein分类
生物科学引用本文复制引用
樊振川,吕玉洁,刘雁霞,王晶,董彬,孟德梅..莱茵衣藻作为绿色细胞工厂生产高附加值 重组蛋白的研究进展[J].天津科技大学学报,2017,32(1):1-12,12.基金项目
国际遗传工程与生物技术中心(ICGEB)研究项目(CRP/CHN15-01) （ICGEB）
