天津科技大学学报2017，Vol.32Issue(1)：13-17,5.DOI:10.13364/j.issn.1672-6510.20160166
整合素integrinβ4调控VEGF诱导的骨髓间充质 干细胞向内皮细胞分化
Integrin β4 Regulating Endothelial Differentiation from Mesenchymal Stem Cells Induced by Vascular Endothelial Growth Factor
摘要
Abstract
Vascular endothelial growth factor(VEGF)can induce mesenchymal stem cells(MSCs)to undergo endothelial cells(ECs)differentiation,but the mechanism of differentiation remains unclear.This study identified that the expression of integrin β4 was upregulated in endothelial differentiation from MSCs to ECs induced by VEGF.At the same time,The CpG-rich promoter regions of integrin β4 were all demethylated by EZ DNA Methylation in MSCs treated with 5-aza-dc and VEGF.When the function-blocking antibodies of integrin β4 were added in the differentiation continuously,the expression of endothelial differentiation marker genes,the endothelial gene kinase insert domain containing receptor(KDR) and Flt-1 were induced.These results suggest that integrin β4 can regulate ECs from MSCs induced by VEGF.关键词
间充质干细胞/内皮细胞/integrinβ4Key words
MSCs/ECs/integrin β4分类
医药卫生引用本文复制引用
张蕊,张同存..整合素integrinβ4调控VEGF诱导的骨髓间充质 干细胞向内皮细胞分化[J].天津科技大学学报,2017,32(1):13-17,5.基金项目
国家自然科学基金资助项目(31171303) （31171303）
