天津科技大学学报2017，Vol.32Issue(1)：25-30,42,7.DOI:10.13364/j.issn.1672-6510.20150225
镰孢霉疫苗表达系统的构建及优化
Construction and Optimization of Vaccine Expression System for Fusarium venenatum
摘要
Abstract
Fusarium venenatum is a newly developed filamentous fungi with a strong protein expression ability.A vaccine expression system was constructed in F.venenatum.With pFGL59 Hyg resistance as the starting plasmid,strong promoters PgpdA,PglaA and Ptrypsin were utilized to construct integrated protein expression vector.This expression system was veri-fied and optimized through expressing the neutralizing epitope protein(COE)of porcine epidemic diarrhea virus.The ex-tracellular protein of the engineered strain was analysed and detected with polyacrylamide gel electrophoresis(SDS-PAGE).The results showed that COE was efficiently expressed and secreted into the extracellular under the control of pro-moter Ptrypsin.The COE protein was not detected in the engineered bacteria extracellular proteins with promoters PgpdA and PglaA.The production of COE protein was obviously increased when coe codons were optimized according to the codon usage preference of F.venenatum.This vaccine expression system provided a new direction for the heterologous expression of vaccine protein in fungi host.关键词
镰孢霉/异源表达/COE蛋白Key words
Fusarium venenatum/heterologous expression/COE protein分类
农业科技引用本文复制引用
何希宏,孙春杰,张华菲,付丽霞,刘浩..镰孢霉疫苗表达系统的构建及优化[J].天津科技大学学报,2017,32(1):25-30,42,7.基金项目
国家自然科学基金资助项目(31201282) （31201282）
天津市科委国际科技合作项目(14RCGHSY00180) （14RCGHSY00180）
