天津中医药2017，Vol.34Issue(3)：181-185,5.DOI:10.11656/j.issn.1672-1519.2017.03.11
基于肿瘤干细胞的西黄丸抗结肠癌作用研究
Antitumor effect of Xihuang pills on colorectal cancer based on cancer stem cells
摘要
Abstract
[Objective] To investigate the effect of Xihuang pills on proliferation, invasion and migration abilities of colorectal cancer stem cells (CSCs) and the expression of multipotent genes of colorectal CSCs. [Methods] CT26 induced colorectal cancer cell Balb/C mice, cancer stem and non-stem cells were primarily obtained. The Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was used to de-termine the proliferation of cancer stem and non-stem cells. The transwell assay was used to determine the effects of Xihuang pills on the invasion and migration abilities of CSCs. qRT-PCR was applied to study the eXpressions of CSCs multipotent genes after treating cells with Xihuang pills. [Results] Xihuang pills can inhibit the proliferation of CSCs and nCSCs effectively, and the effect is dose-dependent. Xi-huang pills have lower IC50 values towards CSCs than nCSCs, which mean it has a more potent direct toxicilty towards CSCs. Further more, it can inhibit the invasion and migration of CSCs, and down-regulate the expression of multipotent genes such as SoX2, Oct4 and Nanog significantly. [Conclusion] Xihuang pills can inhibit the proliferation, invasion and migration abilities of colorectal CSCs effectively, and down-regulate the expression of multipotent genes dramatically. The anti-colorectal effect of Xihuang pills may contribute to its inhibition of CSCs.关键词
西黄丸/肿瘤干细胞/增殖/迁移/多能干基因/结肠癌Key words
Xihuang pills/cancer stem cells/proliferation/migration/multipotent genes/colorectal cancer分类
医药卫生引用本文复制引用
李姗姗,边育红,王丽..基于肿瘤干细胞的西黄丸抗结肠癌作用研究[J].天津中医药,2017,34(3):181-185,5.基金项目
天津市科技支撑计划(12ZCDZSY16800). （12ZCDZSY16800）
