天津中医药2017，Vol.34Issue(3)：190-194,5.DOI:10.11656/j.issn.1672-1519.2017.03.13
快速老化小鼠脑组织Aβ、CypD单核苷酸多态性及"三焦针法"对其作用的研究
Research on the single nucleotide polymorphism of Aβand CypD in brain tissue of senescence accelerated mice and the intervention effect of triple energizer techniques of needling
摘要
Abstract
[Objective] To study the differential eXpression of Aβand CypD in hippocampal and cortical neurons of senescence acceler-ated mice SAMP8 and the same strain normal mice SAMR1 were caused by single nucleotide polymorphism (SNP) in their gene CDs re-gions, DNA sequence, and to study if the triple energizer techniques of needling has intervention effect on DNA sequence of Aβ and CypD CDs regions in hippocampal and cortical neurons of senescence accelerated mice SAMP8 and the same strain normal mice SAMR1. [Methods] Senescence accelerated mice SAMP8 was selected as the model, using PCR amplification and SNP generation sequencing method to compare DNA sequences of Aβand CypD CDs primer design sites in their corteX and hippocampus and to observe intervention effect of triple energizer techniques of needling on DNA sequence of Aβand CypD CDs regions. [Results] SNP were not found in DNA sequences of CDs primer design sites of SAMR1 and SAMP8 mice's corteX and hippocampus, and no changes were found in DNA se-quences of CDs primer design sites of acupuncture group mice's corteX and hippocampus. [Conclusion] No relationship was found be-tween of Aβand cypd mRNA and protein differential eXpression and SNP in DNA sequence of their gene CDs regions and no intervention effect of triple energizer techniques of needling on DNA sequence of Aβand cypd gene CDs regions. Furthermore, it is speculated that there was no relationship between the occurrence of AD and SNP in DNA sequence of Aβand cypd CDs regions and that triple energizer techniques of needling is not by changing the DNA sequence of Aβand cypd CDs regions to regulate mRNA and protein eXpression levels of Aβand cypd.关键词
阿尔茨海默病/单核苷酸多态性/Aβ/CypDKey words
Alzheimer's disease/single nucleotide polymorphism/Aβ/CypD分类
医药卫生引用本文复制引用
韩秀娣,付于..快速老化小鼠脑组织Aβ、CypD单核苷酸多态性及"三焦针法"对其作用的研究[J].天津中医药,2017,34(3):190-194,5.基金项目
国家自然科学基金面上项目(81173414). （81173414）
