天津中医药2017，Vol.34Issue(3)：200-203,4.DOI:10.11656/j.issn.1672-1519.2017.03.15
表儿茶素及其衍生物表儿茶素没食子酸酯通过MAPK-ERK44/42通路抑制前列腺间质细胞增殖
Epicatechin and its derivate Epicatechin gallate inhibit prostatic stromal cell proliferation through selective MAPK-ERK44/42 pathway
摘要
Abstract
[Objective] To study the effect and mechanism of Epicatechin (EC) and its derivate Epicatechin gallate (ECG) in cynomorium songaricum on the proliferation of prostrate stromal cell line WPMY-1. [Methods] WPMY-1 cells were treated with different doses of ECG and EC in vitro. MTT assay was performed to detect the effects of ECG and EC on WPMY-1 cell proliferation. PCR and western blot were performed to detect the mRNA and protein eXpressions of proliferating cell nuclear antigen proliferating cell nuclear antigen(PCNA) separately in WPMY-1 cells treated with ECG or EC. The phosphorylation levels of MAPK signaling pathway ERK44/42, P38, and SAPK/JNK were then detected by western blot in WPMY-1 cells after treatment with ECG or EC. The effects of different MAPK signalings on ECG and EC-suppressed cell proliferation were investigated in WPMY-1 cells after treatment with or without ERK44/42 agonist C6-Ce-ramide (C6) or dual agonist Anisomycin (ANI) of P38, SAPK/JNK. [Results] Both ECG and EC (1 nmol/L to 10μmol/L) inhibited the proliferation of WPMY-1 cells significantly (P<0.01) and PCNA eXpressions in mRNA and protein levels in WPMY-1 cell(P<0.05). the constitutive phosphorylation levels of ERK44/42 and SAPK/JNK were significantly suppressed by ECG and EC, which of P38 was not al-tered. After the adding of C6 blocked ECG and EC-induced suppression of WPMY-1 cell proliferation(P<0.05 or P<0.01). The change of cell proliferation was not significant after the adding of ANI. [Conclusion] ECG and EC inhibit prostatic stromal cell proliferation through ERK44/42 pathway, which provides evidence for their mechanism on benign prostatic hyperplasia prevention.关键词
表儿茶素没食子酸酯/表儿茶素/良性前列腺增生/细胞增殖/MAPK通路Key words
Epicatechin gallate/Epicatechin/benign prostatic hyperplasia/cell proliferation/MAPK pathway分类
医药卫生引用本文复制引用
焦婵媛,景春晖,员小婷,苗琳..表儿茶素及其衍生物表儿茶素没食子酸酯通过MAPK-ERK44/42通路抑制前列腺间质细胞增殖[J].天津中医药,2017,34(3):200-203,4.基金项目
国家自然科学基金青年科学基金项目(81303293) （81303293）
天津市应用基础及前沿技术研究计划青年基金项目(14JCQNJC13400). （14JCQNJC13400）
