生态环境学报2016，Vol.25Issue(12)：1983-1990,8.DOI:10.16258/j.cnki.1674-5906.2016.12.013
一株异养硝化-好氧反硝化细菌的分离鉴定及其脱氮性能研究
Study on Isolation, Identification and Nitrogen Removal Performance of A Heterotrophic Nitrification-aerobic Denitrification Bacterium
摘要
Abstract
Compared with traditional removal of nitrogen, nitrogen removal by heterotrophic nitrification-aerobic denitrification (HNAD) bacteria has several advantages, which has been widely concerned. In this study, Y1, one strain of HNAD bacteria with high nitrogen removal performance was isolated from activated sludge using acetamide as the sole carbon source. According to the results of morphological observation, physiological and biochemical characteristics test and 16S rRNA sequencing, the strain Y1 was identified as Acinetobater johnsonii. The results of physiological and biochemical characteristics test indicated that Y1 was capable of decomposing indole, citrate, hydrogen sulfide and hydrogen peroxide, but couldn't utilize methyl red, glucose, sucrose, gelatin, starch, four methyl para-phenylene diamine hydrochloride and urea. In order to verify the nitrogen removal performance, Y1 was cultivated in heterotrophic nitrification medium and aerobic denitrification medium to measure the concentrations of NH4+-N, NO3--N, NO2--N. During the first 108 hours, 66.9% of the NH4+-N and 100% of the NO3--N in the heterotrophic nitrification medium were removed at a rate of 0.53 and 0.10 mg·L-1·h-1, respectively. During the first 84 hours, 69.7% of the NO3--N in aerobic denitrification medium was removed with a rate of 0.74 mg·L-1·h-1. The results above indicated that Y1 had high nitrogen removal performance. For further study the growth requirements, Y1 was cultured with different carbon and nitrogen sources under the same conditions, the results showed that Y1 grew best and had best nitrogen removal performance in the medium with sodium succinate as the sole carbon source, and Y1 utilized inorganic nitrogen better than organic nitrogen.关键词
异养硝化-好氧反硝化细菌/分离/鉴定/活性污泥/脱氮Key words
heterotrophic nitrification-aerobic denitrification bacteria/screen/identification/activated sludge/nitrogen removal performance分类
资源环境引用本文复制引用
刘小英,冯晟,班宜辉,薛领,林志聪,吕坤媛,徐舟影..一株异养硝化-好氧反硝化细菌的分离鉴定及其脱氮性能研究[J].生态环境学报,2016,25(12):1983-1990,8.基金项目
武汉理工大学国家级大学生创新创业训练计划项目(20161049706007) （20161049706007）
国家自然科学基金项目(31400435 （）
21407114) （）
湖北省自然科学基金项目(2015CFB596) （2015CFB596）
