西安交通大学学报（医学版）2017，Vol.38Issue(2)：210-214,5.DOI:10.7652/jdyxb201702011
宫颈癌细胞系中EMT相关基因的表达及其意义
Expressions of epithelial-mesenchymal transition-associated genes in cervical cancer cell lines
摘要
Abstract
Objective Epithelial-mesenchymal transition (EMT)is thought to be one of the important mechanisms of invasion and metastasis of carcinoma cells,which includes down-regulation of epithelial cell markers and up-regulation of mesenchymal markers.The aim of our study was to investigate whether EMT occurs in cervical cancer cell lines and determine the proper cell model to study the mechanism of EMT in cervical cancer.Methods We detected the mRNA expression of EMT-associated genes,namely,E-cadherin,vimentin,Snail and Slug,in four cervical cancer cell lines using reverse transcriptase-polymerase chain reaction analysis.E-cadherin and vimentin protein expression levels were examined using Western blotting. Snail and Slug protein expression levels were examined by immunocytochemical staining.Results Vimentin and Snail mRNA was expressed in all of the four cell lines.E-cadherin and Slug mRNA was expressed in SiHa,HeLa and RJC-1 cells except CS1213 cells.E-cadherin protein was found in SiHa and RJC-1 cells whereas vimentin protein was detected in HeLa and CS1213.Snail protein was expressed in all of the four cell lines.Slug protein was found in SiHa,HeLa and RJC-1 cells.Conclusion It seems that HeLa and CS1213 display the phenotype of mesenchymal cells whereas SiHa and RJC-1 remain the epithelial phenotype.关键词
宫颈癌/上皮-间质转化(EMT)/E-cadherin/vimentin/Snail/SlugKey words
cervical cancer/epithelial-mesenchymal transition (EMT)/E-cadherin/vimentin/Snail/Slug分类
医药卫生引用本文复制引用
李晓锋,陈葳,李旭,赵乐..宫颈癌细胞系中EMT相关基因的表达及其意义[J].西安交通大学学报（医学版）,2017,38(2):210-214,5.基金项目
国家自然科学基金资助项目(No.30872928) Supported by the National Natural Science Foundation of China (No.30872928) （No.30872928）
