西安交通大学学报（医学版）2017，Vol.38Issue(2)：215-220,6.DOI:10.7652/jdyxb201702012
IL-18基因联合二萜生物碱对舌鳞癌细胞的增殖抑制与促凋亡作用
Effects of IL-18 gene combined with diterpenoid alkaloids in inducing proliferation and promoting apoptosis of tongue squamous carcinoma cells
摘要
Abstract
Objective To investigate the effects of human IL-18 gene combined with diterpenoid alkaloids in inhibiting the proliferation and inducing the apoptosis of tongue squamous carcinoma cells Tscca.Methods We constructed recombinant plasmid pEGFPN3-IL-18 and tranfected it into tongue squamous carcinoma cells Tscca.The transduction efficiency of the target cells was detected by fluorescent microscopy,cytotoxic effect of IL-18 gene with diterpenoid alkaloids on Tscca was detected by MTT assay,and apoptosis was detected by flow cytometry. Western blot was employed to examine the expression level of cellular signal-regulated kinase Akt/p-Akt.Results The tongue squamous cells Tscca which transfected pEGFPN3-IL-18 had significantly increased apoptosis compared with non-transfected cells (P<0 .05 ).Tongue carcinoma squamous cells cultured with diterpenoid alkaloids at the concentrations of 0 .2 ,0 .4 and 0 .6 mg/mL had significantly increased apoptosis in a dose-dependent manner (P<0.05).Human IL-18 gene combined with diterpenoid alkaloids for 48 hours inhibited significantly Tscca in a concentration-dependent manner compared with diterpenoid alkaloids alone (P<0 .05 ).The two in combination could also decrease the protein level of p-Akt dose-dependently.Conclusion The combination of pEGFPN3-IL-18 and diterpenoid alkaloids has a synergistic effect in inhibiting the growth of tongue squamous carcinoma cells Tscca.关键词
白细胞介素18(IL-18)/二萜生物碱/联合用药/舌鳞状细胞癌/增殖抑制/凋亡Key words
interleukin-18 (IL-18 )/diterpenoid alkaloid/combination therapy/squamous cell carcinoma/inhibition of proliferation/apoptosis分类
医药卫生引用本文复制引用
胡雅琳,侯美玲,郑文娇,张泽南,何韶衡,李志刚..IL-18基因联合二萜生物碱对舌鳞癌细胞的增殖抑制与促凋亡作用[J].西安交通大学学报（医学版）,2017,38(2):215-220,6.基金项目
辽宁医学院校长基金-奥鸿博泽研究生科技创新基金(No.AH2015007) The President Fund of Liaoning Medical College-Ao Hong Bo Ze Graduate Technology Innovation Fund (No.AH2015007) （No.AH2015007）
