西安交通大学学报（医学版）2017，Vol.38Issue(2)：226-230,5.DOI:10.7652/jdyxb201702014
miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭
miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1
摘要
Abstract
Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by down-regulating FOXM1.Methods miR-498 mimic and miR-NC were transfected into A549 cells.Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells;Western blot was used to detect the expressions of COL1A1,COL1A5 and FOXM1 in A549 cells.Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498.Results Compared with those in the control group,the expressions of COL1A1,COL1A5 and FOXM1 were decreased,and the migration and invasion abilities of A549 cells were decreased in the miR-498 group (both P<0 .01 ).The luciferase activity of the FOXM1-3′-UTR plasmid was significantly suppressed by miR-498 (P<0 .05 );over-expression of FOXM1 could reverse the effect of miR-498 on A549 cells.Conclusion miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1.关键词
肺腺癌/微小RNA498(miR-498)/COL1A1/COL1A5/FOXM1/侵袭Key words
lung adenocarcinoma/miR-498/COL1A1/COL1A5/FOXM1/invasion分类
医药卫生引用本文复制引用
唐曦,胡娅,徐炎华,汪春林,邱萍,王向辉..miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭[J].西安交通大学学报（医学版）,2017,38(2):226-230,5.基金项目
湖北省教育厅项目(No.B2013280) （No.B2013280）
湖北省卫生厅项目(No.2012Z-Y31) Supported by Project of Hubei Department of Education (No.B2013280)and Project of Hubei Department of Health (No.2012Z-Y31) （No.2012Z-Y31）
