西安交通大学学报（医学版）2017，Vol.38Issue(2)：290-294,5.DOI:10.7652/jdyxb201702025
盐酸青藤碱诱导人肝癌细胞凋亡及其对IL-1β,TNF及C/EBPβ水平的影响
Sinomenine hydrochloride induces the apoptosis of hepatocellular carcinoma SMMC-7721 cells and its effect on expression levels of IL-1β,TNF and C/EBPβ
摘要
Abstract
Objective To observe the expression levels of IL-1β,TNF and C/EBPβduring the process of apoptosis induced by sinomenine Hydrochloride in hepatocellular carcinoma SMMC-7721 cells to clarify sinomenine hydrochloride'anti-tumor efficacy and its mechanism.Methods MTT assay was used to determine the in vitro effect of sinomenine hydrochloride on the growth of hepatocellular carcinoma SMMC7721 cells cultured.In invitro cultured human hepatocellular SMMC7721 cells,quantitative analysis of apoptotic cells induced by sinomenine hydrochloride were assessed by flow cytometry after AnnexiV-FITC/PI staining. ELISA method was used to determine the expression levels of IL-1βand TNF-α,SMMC7721 cells were cultured for 48 hours with sinomenine hydrochloride 2 mmol/L,then the effects of sinomenine hydrochloride on C/EBPβwere detected by Western blot. Results Sinomenine hydrochloride inhibited the invitro growth of human hepatocellular carcinoma SMMC 7721 cells in concentration-and time-dependent manners as determined by MTT assay (P<0 .05 ). After treatment with sinomenine hydrochloride of different concentrations (0 ,0 .5 ,1 and 2 mmol/L),the apoptosis rate of the cells was (8.31±0.81,12.62±1.13,27.43±0.97,and 51.74±0.85)%,with significant difference between the experiment groups and control group (P<0.05).The secretions of TNF-αand IL-1βwere decreased in supernatant of human hepatocellular carcinoma SMMC7721 cells.Sinomenine hydrochloride could significantly increase the C/EBPβprotein expression level (P<0.05 ).Conclusion Sinomenine hydrochloride inhibited the in vitro growth of human hepatocellar carcinoma SMMC7721 cells through apoptotic induction.This may be related to its decreasing the expression levels of IL-1β,TNF and C/EBPβand destroying tumor microenvironment during the process of apoptosis induced by dinomenine hydrochloride in hepatocellular varcinoma SMMC7721 cells.关键词
肝细胞肝癌/IL-1β/TNF-α/C/EBPβ/盐酸青藤碱/凋亡Key words
hepatocellular carcinoma/IL-1β/TNF-α/C/EBPβ/sinomenine hydrochloride/apoptosis分类
医药卫生引用本文复制引用
曹瑞琪,卢新兰,任牡丹,张俊,卢桂芳,张丹,赵艳,闫红林,和水祥..盐酸青藤碱诱导人肝癌细胞凋亡及其对IL-1β,TNF及C/EBPβ水平的影响[J].西安交通大学学报（医学版）,2017,38(2):290-294,5.基金项目
国家自然科学基金面上项目(No.81502095) （No.81502095）
陕西省教育厅专项科研计划项目(No.2013JK0786) （No.2013JK0786）
陕西省社会发展科技攻关项目(No. 2015SF55) Supported by the Youth Project of the National Natural Science Foundation of China (No.81502095),the Special Research Project of Education Department of Shaanxi Province (No.2013JK0786 ),and the Social Development,Scientific and Technological Research Project of Shaanxi Province (No.2015SF55) （No. 2015SF55）
