西北林学院学报2017，Vol.32Issue(2)：112-116,160,6.DOI:10.3969/j.issn.1001-7461.2017.02.19
美洲黑杨×青杨派杂种无性系SSR指纹图谱的构建与遗传差异性分析
Construction of Fingerprint and Analysis of Genetic Difference for Hybrid Clones of Populus deltoides × Section tacamahaca Using SSR Marker
摘要
Abstract
DNA fingerprints of 9 hybrid clones of Populus deltoides × Section tacamahaca and 3 parents were established by using SSR (simple sequence repeats) marker,and differences in genetic relationships were analyzed.The results showed that 94 clear bands were generated from 12 pairs of primers selected and each primer amplified 7.8 bands,in which the polymorphic bands were 88 and the polymorphic rate was 90.4％.The similarity coefficients of the Populus clones ranged from 0.44 to 0.80 with an average of 0.62.Those polymorphic bands were used for establishing fingerprint of 12 clones.The clustering analysis showed that the genetic differences between those hybrid clones and female parent (P.deltoides) were low.Each clone had its own unique bands,which showed that different experimental materials had certain genetic differences in DNA level,which could differentiate them.关键词
杨树/无性系/分子标记/SSR/指纹图谱Key words
Populus/clone/molecular marker/SSR/fingerprint分类
农业科技引用本文复制引用
藕丹,樊军锋,周永学,高建社..美洲黑杨×青杨派杂种无性系SSR指纹图谱的构建与遗传差异性分析[J].西北林学院学报,2017,32(2):112-116,160,6.基金项目
国家林业局林业公益性行业科研专项(201404113). （201404113）
