西北林学院学报2017，Vol.32Issue(2)：130-135,6.DOI:10.3969/j.issn.1001-7461.2017.02.22
青海地方核桃种质资源遗传多样性研究
Genetic Diversity of Germplasm Resources of Walnut in Qinghai
摘要
Abstract
The paper aimed to analyze the genetic diversity of walnut in Qinghai by ISSR molecular marker in 222 walnut leaf samples collected from deferent eco-geographical regions in Qinghai.The results showed that 167 bands were amplified by 12 primers,of which 127 were polymorphic loci.At species level,the number of polymorphic loic was 127,the percentage of polymorphic loci was 76.05％,observed number of alleles was 1.760 5,effective alleles was 1.384 9,Nei's gene diversity was 0.233 2,Shannon's Information index(Ⅰ) was 0.357 7.Genetic coefficient of differentiation among populations based Nei's gene diversity was 0.081 7.It showed that 8.26％ genetic differentiation existed among populations,the genetic differentiation within populations was higher than that among populations.The mean value of genetic distance was 0.025 7,estimation of gene flow from Gst was 5.619 8.It indicated that there was a large gene exchange among the populations of walnut in Qinghai.Analysis of AMOVA also demonstrated that genetic variance among populations was larger than within a population.关键词
青海核桃/ISSR分子标记/遗传多样性Key words
Qinghai walnut/ISSR molecular marker/genetic diversity分类
农业科技引用本文复制引用
刘宝尧,刘小利,魏海斌,顾文毅..青海地方核桃种质资源遗传多样性研究[J].西北林学院学报,2017,32(2):130-135,6.基金项目
青海省科技成果转化专项“优质早实薄皮核桃丰产栽培技术集成与推广”(2016-NK-133). （2016-NK-133）
