西北林学院学报2017，Vol.32Issue(2)：136-142,7.DOI:10.3969/j.issn.1001-7461.2017.02.23
3种不同地理起源葡萄种质资源的抗旱性比较
Comparison of Drought Tolerance of Three Grape Germplasm Resources from Different Geographic Origins
摘要
Abstract
The objective of this study was to examine the differences in drought resistance of three grapevines from different geographic origins,including Vitis yeshanensis accession ' Yanshan-1 ' (Y),Vitis riparia accession ' He'an (♀) ' (H) and Vitis vinifera cv.'Red Globe' (R).The plantlets of the three were used as materials,the changes of morphology,proline,MDA,the activities of protective enzymes and superoxide anion free radicals contents in the blades were investigated under the stress of PEG-6000.The occurrence of the dry spots in the blades was different among the three:the spots appeared 3,12 and 27 hours after the stress for H,R,and Y,respectively.With the increase of drought stress time,H was injured mostly,followed by R,and Y was slightly injured.The proline contents in the blades of the three increased with time.Seventy-two hours after the stress,the proline content in the blades of Y was 1.14 and 4.48 folds to R and H,respectively.The activities of POD,CAT and SOD initially presented an upward trend but all declined later.Seventy-two hours after the stress,the POD activity of Y was 6.2 and 49.2 folds to R and H,the CAT activity of Y was 0.44 and 1.47 folds R and H,and the SOD activity of Y was 1.95 and 1.05 folds to R and H,respectively.The superoxide anion free radicals content initially increased,and then decreased.At 72 h after the stress,the superoxide anion free radicals content of Y was 1.9 and 3.0 folds to R and H,respectively.The MDA content initially increased,but declined later.At 72 h after stress,the MDA content of Y was 1.4 and 1.26 folds to R and H,respectively.Considering morphological changes and the changes of the physiological and biochemical indexes comprehensively,Y demonstrated the highest drought resistance,followed by R and H.关键词
葡萄/种质资源/试管苗/聚乙二醇(PEG-6000)/干旱胁迫/抗旱性Key words
Vitis/germplasm resource/tube seedling/PEG-6000/drought stress/drought tolerance分类
农业科技引用本文复制引用
薛加楠,崔小月,张琪,吴梦晓,张剑侠..3种不同地理起源葡萄种质资源的抗旱性比较[J].西北林学院学报,2017,32(2):136-142,7.基金项目
国家科技支撑计划子课题(2013BAD02B04-06). （2013BAD02B04-06）
