西北林学院学报2017,Vol.32Issue(2):205-211,7.DOI:10.3969/j.issn.1001-7461.2017.02.35
四川九龙山自然保护区林麝栖息地典型植被土壤微生物群落特征
Microbial Community Characteristics of Typical Vegetation Soil at Forest Musk Deer Habitats in Sichuan Jiulong Mountain Nature Reserve
摘要
Abstract
The objectives of this study were to explore the characteristics of soil microbial communities in the soils of typical vegetations (shrub forest,mixed coniferous and broad-leaved mixed forest) at forest musk deer habitat in Sichuan Jiulong Mountain Nature Reserve,and to examine the effects of soil microbials on the composition of ground vegetations.The number of soil colonies was determined by plate colony dilution method.Isolated soil bacteria and actinomycetes were identified by 16S rRNA PCR method.Soil physicochemical properties were determined by the conventional methods.Eleven species of microorganisms were identified.They were mainly consisted of Bacillus weihenstephanensis,B.megaterium and B.thuringiensis.Among the microorganisms isolated from the soil of three vegetation types,the number of bacteria (over 70 %) represented absolute advantage,actinomycetes were followed,and no fungi were separated.Distribution trend in three soil types of microorganisms in the soil profile was 0-20>20-40>40-60 cm.The correlation analysis indicated that pH value was significantly and positively correlated to actinomycetes (R=0.676,P<0.05).Total microorganisms were significantly and positively correlated to total bacteria (R=1,P<0.01).The correlationship between total microorganismsl and actinomycetes was significant and positive (R=1,P<0.05).关键词
九龙山自然保护区/林麝栖息地/土壤化学性质/土壤微生物Key words
Jiulong Mountain Nature Reserve/forest musk deer habitat/soil physicochemical property/soil microorganism分类
农业科技引用本文复制引用
程建国,纪昌海,罗燕,周鑫..四川九龙山自然保护区林麝栖息地典型植被土壤微生物群落特征[J].西北林学院学报,2017,32(2):205-211,7.基金项目
四川省林业厅省级自然保护区重点物种科学研究项目 ()
四川农业大学双支项目. ()