现代妇产科进展2017，Vol.26Issue(3)：165-168,4.DOI:10.13283/j.cnki.xdfckjz.2017.03.001
宫颈癌细胞分泌外泌体介导上皮-间质转化提高癌前细胞侵袭能力的体外研究
Exosomes derived from cervical carcinoma cells promote preneoplastic cell invasion in vitro via epithelial mesenchymal transition
摘要
Abstract
Objective:To investigate whether Exosomes secreted by cervical cancer cell lines Siha ( Siha-Exo) have ability to promote Ect1 invasion via epithelial mesenchymal transi-tion ( EMT) in cervical cancer. Methods:Morphology and marker protein of Siha-Exo isolated by different ultracentrifugation were identified by transmission electron microscopy and Western blot. And green fluorescent PKH67-labeled Siha-Exo detected by confocal images for biological activity. Ect1 was co-cultured with Siha-Exo or PBS for 48 hours. The expression of epithelial and mesenchyme markers were examined by Western blot. Transwell chambers were used in vitro invasion assay. Results:Siha-Exo exhibited typical cup-shaped vesicles and a size range of 30-100nm,expressed exosomal marker protein CD63 and CD81,and were biologically uptaked by recipient Ect1. Compared with PBS treated cells,Ect1 treated with Siha-Exo decreased ex-pression of epithelial marker E-Cadherin andβ-Catenin,and increased expression of mesenchy-mal marker N-Cadherin and Vimentin. Meanwhile in Siha-Exo group,numbers of Ect1 penetrat-ing transwell membrane significantly increased in contrast with PBS group ( P=0 . 01 ) . Conclu-sion:Siha-Exo elicited Ect1 to an EMT-like phenotype,thus promoting invasion in vitro.关键词
外泌体/宫颈癌/上皮间质转化/侵袭Key words
Exosomes/Cervical cancer/Epithelial mesenchymal transition/Invasion分类
医药卫生引用本文复制引用
易红艳,陈春林,钟梅,余艳红,周琛斐,梁莉,王薇,魏文斐,陈晓静,吴湘光,严瑞明,刘国炳..宫颈癌细胞分泌外泌体介导上皮-间质转化提高癌前细胞侵袭能力的体外研究[J].现代妇产科进展,2017,26(3):165-168,4.基金项目
国家自然科学基金(No:81072132 （）
81372871 （）
81672589 （）
JCYJ20160429161218745) （）
