现代妇产科进展2017，Vol.26Issue(3)：174-178,5.DOI:10.13283/j.cnki.xdfckjz.2017.03.003
缺氧微环境下HIF-1α对卵巢癌A2780细胞侵袭转移的影响及分子机制
Effect of HIF-1αon the invasion of ovarian cancer A2780 cell under hypoxic condition
摘要
Abstract
Objective:To explore the probable role of HIF-1α in the invasion and me-tastasis of ovarian cancer A2780 cell under hypoxic condition. Methods:Ovarian cancer A2780 cell were cultured under normoxic condition and hypoxic environment respectively. The mRNA and protein expressions of HIF-1α and MMP13 were detected by RT-PCR and Western blot. The effects of siRNA against HIF-1α on MMP13 expression were examined by RT-PCR and Western blot. Transwell invasion assays were performed to test the invasive ability of A2780 cells. Results:After exposure to hypoxia,mRNA and protein levels of HIF-1αand MMP13 were up-regulated. SiRNA inhibited HIF-1α expression effectively,which also inhibited MMP13 ex-pression. The number of invading A2780 cells decreased after HIF-1α was silenced. Conclu-sion:HIF-1α promotes ovarian cancer cell invasion through a MMP13 mechanism. It might be an effective strategy targeting HIF-1α-MMP13 to inhibit the invasion and metastasis of ovarian cancer.关键词
卵巢癌/缺氧诱导因子-1α/基质金属蛋白酶13/侵袭转移Key words
Ovarian cancer/HIF-1α/MMP13/Invasion and metastasis分类
医药卫生引用本文复制引用
施宣忍,刘群,王莉,崔晶..缺氧微环境下HIF-1α对卵巢癌A2780细胞侵袭转移的影响及分子机制[J].现代妇产科进展,2017,26(3):174-178,5.基金项目
国家自然科学基金(No:81301789)和山东省自然科学基金(No:ZR2011HQ013) （No:81301789）
