现代妇产科进展2017，Vol.26Issue(3)：186-190,5.DOI:10.13283/j.cnki.xdfckjz.2017.03.030
hUCMSCs移植提高围绝经期大鼠血清雌激素水平的持续时间及对卵巢P450arom表达的影响
The effect of hUCMSCs transplantation on the duration of serum estrogen level and ovarian P450arom expression of perimenopausal rats
摘要
Abstract
Objective:To investigate the effect of human umbilical cord mesenchymal stem cells ( hUCMSCs) transplantation on the duration of serum estrogen ( E2 ) level and its effect on the expression of aromatase cytochrome P450 (P450arom) in the perimenopausal rats. Methods:The 30 natural aging female Sprague-Dawley rats (12~14 months old) of SPF class were selected through vaginal smears, which were randomly divided into A and B groups. We selected 15 young female SD rats (3~5 months old) with fertility as C group at the same time. Group B was transplanted 1ml hUCMSCs suspension at the concentration of 1 × 106 cells/ml through tail vein. After 48 hours,group B was again injected with 1ml hUCMSCs suspension. Group A was injected with 1ml PBS per rat via the tail vein at the same time. Group C were ac-cepted no any treatments. Before transplantation,1ml of blood was gathered from each rat's or-bital. At 14,21 and 28 days after the final cell transplantation,the five rats from each group were selected randomly and euthanized. Blood was taken from orbital and ovaries were removed for ELISA analysis of E2 levels, immunohistochemistry and Western blot detection of ovarian P450arom expression. Results:Serum level of E2 and ovarian expression of P450arom were in-creased in group B at time-points of 14 ,21 ,28 days after transplantation ( P<0 . 05 ) . The level of E2 and ovarian expression of P450arom had no significant difference in group B at three time-points(P>0. 05). Conclusion:hUCMSCs improve serum E2 level of perimenopausal rats at least 28 days after transplantation. HUCMSCs transplantation may improve E2 level of perimeno-pausal rats via promoting ovarian P450arom expression.关键词
hUCMSCs移植/围绝经期/雌激素/细胞色素P450芳香化酶Key words
hUCMSCs transplantation/Perimenopausal/Eestrogen/Aromatase cyto-chrome P450分类
医药卫生引用本文复制引用
李嘉,尹春艳..hUCMSCs移植提高围绝经期大鼠血清雌激素水平的持续时间及对卵巢P450arom表达的影响[J].现代妇产科进展,2017,26(3):186-190,5.基金项目
广东省医学科学技术研究基金(No:A2016497) （No:A2016497）
