中国组织工程研究2017，Vol.21Issue(4)：493-498,6.DOI:10.3969/j.issn.2095-4344.2017.04.001
黄精多糖不依赖于LRP5激活信号通路调控成骨细胞分化
Polygonatum sibiricum polysaccharide promotes osteogenesis by signaling pathway activation after LRP5 silencing
摘要
Abstract
BACKGROUND:Our previous studies have found that polygonatum sibiricum polysaccharide (PSP) promotes osteogenic differentiation of bone marrow mesenchymal stem cel s (BMSCs) by Wnt/β-catenin signaling pathway, but the molecular mechanism is unclear.OBJECTIVE:To investigate the effect of PSP promoting the osteogenic differentiation via Wnt signaling pathways in BMSCs after LRP5 silencing. METHODS:LRP5 interference vectors were constructed and then transfected into C57BL/6 mouse BMSCs cultured in vitro. The transfection efficiency of cel s was calculated under fluorescence inverted microscope and the expression of LRP5 protein was detected by western blot assay. The osteogenic potential of BMSCs after LRP5-siRNA transfection was analyzed by alkaline phosphatase staining, alizarin red staining and western blot assay. Effect of PSP on the osteogenic differentiation of LIRP5-silenced mouse BMSCs was detected by real-time PCR and dual luciferase assay. RESULTS AND CONCLUSION:Compared with the control group, the mineralization ability, the mRNA expressions of Runx2 and Osterix, and the protein expression of LRP5 were significantly decreased in the LRP5-siRNA group (P<0.05). PSP could promote LRP5-siRNA transfected mouse BMSCs differentiating into osteoblasts and significantly upregulated the expressions ofβ-catenin and Osterixin, and also induced the high expression of luciferase reporter gene (TOPFlash) containing wild type TCF binding sites (P<0.05). To conclude, LRP5 plays an important role in the process of mouse BMSCs differentiating into osteoblasts. PSP can promote the osteogenic differentiation of mouse BMSCs by activating the Wnt/β-catenin signaling pathway independent on LRP5.关键词
组织构建/骨细胞/黄精多糖/骨髓间充质干细胞/成骨分化/Wnt/β-catenin通路/病毒转染/国家自然科学基金分类
医药卫生引用本文复制引用
彭小明,宗少晖,曾高峰,农梦妮,杜力,李珂珂,何基琛,施雄智,吴云乐..黄精多糖不依赖于LRP5激活信号通路调控成骨细胞分化[J].中国组织工程研究,2017,21(4):493-498,6.基金项目
国家自然科学基金资助项目(81360279) （81360279）
