中国组织工程研究2017，Vol.21Issue(4)：520-526,7.DOI:10.3969/j.issn.2095-4344.2017.04.005
生长因子对椎间盘终板软骨细胞抗氧化能力的影响
Effects of different growth factors on the antioxidant capacity of endplate chondrocytes
摘要
Abstract
BACKGROUND:It is wel-known that vitamin E holds antioxidant capacity, but whether other growth factors have the same effect on endplate chondrocytes has not yet been reported. OBJECTIVE:To observe the effect of different growth factors on the antioxidant ability of endplate chondrocytes in the intervertebral disc. METHODS:Endplate chondrocytes were primary cultured, and then divided into four groups, including blank control, serum deprivation, hydrogen peroxide stimulation and hydrogen peroxide stimulation combined with different growth factors groups. The 4th group was subdivided into insulin-like growth factor-1, basic fibroblast growth factor, transforming growth factorβ, forskolin and vitamin E groups. The expression levels of caspase-3, matrix metal oproteinase 13 and 3, inhibitor of metal oproteinase 1 as wel as thrombin 4 and 5 were detected by real-time PCR. Cel apoptosis was analyzed through apoptosis kit and flow cytometry. Cel synthesis and secretion were detected by western blot assay. The total antioxidant capacity and the hydrogen peroxide content were determined by kit, and then statistical y analyzed. RESULTS AND CONCLUSION:Different growth factors had significant differences in the endplate chondrocyte apoptosis, secretion and antioxidant capacity. To conclude, transforming growth factorβand forskolin do further damage to the cel s stimulated by hydrogen peroxide, while insulin-like growth factor-1 and vitamin E expose protective effect on the injured cel s.关键词
组织构建/软骨细胞/胰岛素生长因子1/碱性成纤维细胞生长因子/转化生长因子/Forskolin/维生素E/椎间盘/终板软骨细胞/总抗氧化能力/过氧化氢/国家自然科学基金分类
医药卫生引用本文复制引用
黄晓东,邓国英,王伟恒,徐立璋,马俊,叶晓健..生长因子对椎间盘终板软骨细胞抗氧化能力的影响[J].中国组织工程研究,2017,21(4):520-526,7.基金项目
国家自然科学基金资助项目(81472071)；国家高技术研究发展计划(863)资助项目(2013AA032203) （81472071）
