中国组织工程研究2017，Vol.21Issue(4)：545-550,6.DOI:10.3969/j.issn.2095-4344.2017.04.009
口腔颌面组织工程血管的初步构建
Preliminary construction of the tissue-engineered oral and maxillofacial vessel
摘要
Abstract
BACKGROUND:Insufficient blood supply after major bone defects leads to osteocyte necrosis;therefore, the tissue-engineered bone cannot meet the clinical requirements. OBJECTIVE:To construct an artificial vessel using in vitro cultured rabbit bone marrow stromal stem cel s and human umbilical vein endothelial cel s combined with the rabbit aortic decel ularized vascular matrix. METHODS:Rabbit bone marrow stromal stem cel s were cultured by adherent method, human umbilical vein endothelial cel s were cultured in vitro and isolated, and rabbit aortic decel ularized vascular stent was prepared. The stent-cel composite was constructed, and its biocompatibility was observed under scanning electron microscope. There were three groups:blank control, control (bone marrow stromal stem cel-stent) and experimental (stent-cel composites) groups (n=10 per group), fol owed by implantation around the smal vessels. Three months later, the vascular samples were col ected for gross observation and hematoxylin-eosin staining. RESULTS AND CONCLUSION:Fibroblast-like cel clones were formed by adherent method, and were positive for CD44+and STRO-1+after climbing;calcified nodule formed in the ossification. Flow cytometry showed CD34+accounting for 43.83%, and visible VIII factor in the endothelial cel s. In the experimental group, endothelial cel s formed a single cel layer onto the scaffold under scanning electron microscope;the artificial vessel grew wel and tubular structure presented and eight angiod structures were found. Thin film-like vessels formed in the blank control group and there were only endothelial-like tissues in the control group. These results suggest that angiod structures can form after the stent-cel composite implantation, causing no immune reaction, but the artificial vessel is visible in absence of muscular layer.关键词
组织构建/组织工程/间质干细胞/内皮细胞/人工血管工程分类
医药卫生引用本文复制引用
杨兴华,彭琳,韩晓燕,陈岱韵..口腔颌面组织工程血管的初步构建[J].中国组织工程研究,2017,21(4):545-550,6.基金项目
山东省教育厅立项项目(J13LL01)；山东省卫生厅立项项目(2009HW094) （J13LL01）
