中国组织工程研究2017，Vol.21Issue(4)：551-558,8.DOI:10.3969/j.issn.2095-4344.2017.04.010
人牙周膜成纤维细胞凋亡与体外高糖环境和脂多糖的交互作用
Interaction of high glucose and lipopolysaccharide on the apoptosis of human periodontal ligament fibroblasts in vitro
摘要
Abstract
BACKGROUND:Both high glucose and lipopolysaccharide have been proved to promote the apoptosis of human periodontal ligament fibroblasts (HPLFs), but their interactions on the HPLF apoptosis in vitro have not yet been reported. OBJECTIVE:To investigate the effect of different concentrations of lipopolysaccharide and high glucose on the proliferation, apoptosis and the expression levels of Bax and Bcl-2 in HPLFs in vitro. METHODS:The primarily cultured HPLFs were identified. The 5-8 generations of HPLFs were col ected and used in the subsequent experiment. The HPLFs were cultured in different concentrations of glucose (5.5 and 25 mmol/L) and lipopolysaccharide (0, 1 and 10 mg/L) for 24 and 48 hours, respectively. RESULTS AND CONCLUSION:Lipopolysaccharide (10 mg/L) could significantly inhibit the cel proliferation, promote the cel apoptosis, upregulate the expression levels of Bax and Bcl-2 mRNA and induce a significant decrease in Bcl-2/Bax ratio in the cel s cultured with 5.5 mmol/L glucose (P<0.05). The lipopolysaccharide-induced suppression of cel proliferation, cel apoptosis, the expressions of Bax and Bcl-2 mRNA as wel as decrease in Bcl-2/Bax ratio were significantly strengthened in the HPLFs treated with 25 mmol/L glucose (P<0.05). Analysis of variance found that high glucose and lipopolysaccharide had a significant interaction on the cel apoptosis (P<0.05). These results reveal that lipopolysaccharide-induced suppression of cel proliferation, cel apoptosis and the expressions of Bax and Bcl-2 mRNA are augmented in HPLFs cultured under high glucose condition, indicating lipopolysaccharide and high glucose interactively act in inducing cel apoptosis.关键词
组织构建/组织工程/高糖/脂多糖/人牙周膜成纤维细胞/凋亡/Bax/Bcl-2/原代培养分类
医药卫生引用本文复制引用
荆冉,郭大伟,廖奕翔,任伟伟,仇静,陈书兰..人牙周膜成纤维细胞凋亡与体外高糖环境和脂多糖的交互作用[J].中国组织工程研究,2017,21(4):551-558,8.基金项目
山东省医药卫生科技发展计划项目(2013WS0014) （2013WS0014）
