中国组织工程研究2017，Vol.21Issue(4)：569-573,5.DOI:10.3969/j.issn.2095-4344.2017.04.013
腰椎间盘突出症推拿治疗前后血清蛋白质组学分析
Serum proteome analysis of lumbar disc herniation before and after massage therapy
摘要
Abstract
BACKGROUND:Understanding the actions and underlying mechanisms of proteins in cel s and organisms and studies on the lumbar disc herniation (LDH)-associated proteins contribute to further clarify the LDH pathogenesis. OBJECTIVE:To analyze the serum proteome changes in LDH patients using two-dimensional gel electrophoresis-tandem mass spectrometry, and to screen the biomarkers for LDH diagnosis and treatment. METHODS:Twenty-five LDH patients were enrol ed and their serums were col ected before and after treatment. After removal of high abundant protein, the serum protein spectrum was compared after two-dimensional gel electrophoresis serum protein spectrum diagram, to look for differential protein spots. Subsequently, the differential protein spots were identified by MALDI-TOF/TOF technique combined with biology software and database retrieval. RESULTS AND CONCLUSION:Six differential protein spots were screened preliminarily, and three kinds of proteins were confirmed after mass spectrum detection, among which, acid glycoprotein was related to the LDH immune regulation and occurrence, development and targeting sites of chemical radiculitis. The expression level of acid glycoprotein was significantly decreased in LDH patients after treatment (P<0.05). These results suggest that acid glycoprotein is associated with LDH through gel electrophoresis-base proteome analysis.关键词
组织构建/组织工程/腰椎间盘突出症/双向电泳/蛋白质组学分类
医药卫生引用本文复制引用
张明顺,关斌辉,范宏元,赵化冰,向开维..腰椎间盘突出症推拿治疗前后血清蛋白质组学分析[J].中国组织工程研究,2017,21(4):569-573,5.基金项目
贵州省科技厅项目(黔科合中药字[2010]LKZ7037)，课题名称节段定位斜扳法治疗腰椎间盘突出症血清蛋白质组学的水平变化及临床研究 （黔科合中药字[2010]LKZ7037）
