中国组织工程研究2017，Vol.38Issue(5)：701-706,6.DOI:10.3969/j.issn.2095-4344.2017.05.008
牙髓干细胞移植治疗缺氧缺血性脑损伤新生大鼠远期行为学及环磷酸腺苷反应元件结合蛋白的变化
Effects of dental pulp stem cell transplantation on the long-term behavior and cAMP response element binding protein in neonatal rats with hypoxic ischemic brain damage
摘要
Abstract
BACKGROUND:cAMP response element binding protein (CREB) is a key protein of memory, which is closely related to long-term memory. It wil provide a new way for the treatment of hypoxic ischemic brain damage (HIBD) to study the effects of dental pulp stem cel s transplantation on the long-term behavior and CREB protein via the lateral ventricle in neonatal HIBD rats. OBJECTIVE:To observe the changes in long-term behavior and CREB protein expression in neonatal HIBD rats after human dental pulp stem cel transplantation, thereby providing scientific evidence for clinical treatment of neonatal HIBD. METHODS:Thirty-six healthy 7-day-old Sprague-Dawley rats were randomly divided into normal, HIBD and cel transplantation group. The hypoxic ischemic brain damage models were established in the brain damage and cel transplantation groups. Twenty-four hours after HIBD, human dental pulp stem cel s were injected into the left lateral cerebral ventricle of rats in the cel transplantation group, total y 3×106 living cel s. Equal volume of normal saline was injected into the left lateral cerebral ventricle of rats in the normal control and HIBD groups. RESULTS AND CONCLUSION:The average time to seek water, the average escape latency and escape distance of the human dental pulp stem cel s group were significantly shorter than those of hypoxic ischemic brain injury group (P<0.01), but longer than those in the normal group (P<0.01). Nissl staining showed that the cel s in the hippocampal CA1 region in human dental pulp stem cel s group were more regular, the number of cel s was significantly higher than that of hypoxic ischemic brain injury group, but stil significantly less than that in the normal group (P<0.05). Immunohistochemical staining results showed that the number of CREB positive cel s in human dental pulp stem cel s group was significantly higher than those in HIBD group, but stil significantly less than those in the normal group (P<0.01). It is suggested that human dental pulp stem cel s transplantation could promote the expression of CREB protein in the hippocampal CA1 region, to improve the long-term learning and memory ability of hypoxic ischemic neonatal rats, and thus repair HIBD.关键词
干细胞/移植/牙髓干细胞/缺氧缺血性脑损伤/环磷酸腺苷反应元件结合蛋白/八臂迷宫/Morris水迷宫/尼氏染色/免疫组织化学染色/新生大鼠/国家自然科学基金分类
医药卫生引用本文复制引用
王爱,牟青杰,王晓莉,闫少珍,曲鹏宇,王海宇,胡温庭..牙髓干细胞移植治疗缺氧缺血性脑损伤新生大鼠远期行为学及环磷酸腺苷反应元件结合蛋白的变化[J].中国组织工程研究,2017,38(5):701-706,6.基金项目
国家自然科学基金项目(81000268)；山东省自然科学基金项目(ZR2014JL049)；山东省医药卫生科技发展计划项目(2014WS0473)；山东自然科学基金项目(ZR2014HQ077)Funding:the National Natural Science Foundation of China, No.81000268 （81000268）
the Natural Science Foundation of Shandong Province, No. ZR2014JL049, ZR2014HQ077 （）
the Medicine and Health Science Development Plan of Shandong Province, No.2014WS0473 （）
