中国组织工程研究2017，Vol.38Issue(5)：707-712,6.DOI:10.3969/j.issn.2095-4344.2017.05.009
尾静脉移植羊膜间充质干细胞对缺血再灌注损伤后神经功能恢复的影响
Effect of human amniotic mesenchymal stem cells transplantation via tail vein on neurological function recovery from ischemic reperfusion injury
摘要
Abstract
BACKGROUND:Studies have shown that overexpression of myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp) is the main reason for early neuronal regeneration failure. OBJECTIVE:To study the effect of human amniotic mesenchymal stem cel s (hAMSCs) transplantation on neural functional recovery of rats with ischemia/reperfusion (I/R) injury. METHODS:Sixty Sprague-Dawley were randomized into sham, I/R, and hAMSCs groups (n=20 per group). Rats in the hAMSCs group were given 1 mL of hAMSCs suspension (1.0×108/L) via the tail vein 24 hours after I/R injury. Rat neurological function recovery was assessed based on behavior changes, as determined by Longa behavioral score, cylinder test, horizontal ladder walking test and limb symmetry test at 1, 3 days, and 1, 2, 3 weeks post transplantation. Cel migration and distribution were observed using immunofluorescence method at 1 day, and 1, 2, 3 weeks post transplantation. MAG and OMgp protein expression was detected by western blot assay at 2 weeks post transplantation. Neuronal apoptosis was detected by TUNEL staining at 3 weeks post transplantation. RESULTS AND CONCLUSION:In the hAMSCs transplantation group, red marker-positive cel s were visible around the injury region at 1 week after transplantation, and over time, these cel s were increased in number. Significant improvement in the neurological function of rats were observed in the hAMSCs group as compared with the I/R group at 3 days and 1, 2, 3 weeks after transplantation (P<0.05), and the expression of MAG and OMgp proteins were also decreased dramatical y in the hAMSCs group (P<0.05). After I/R injury, the number of apoptotic cel s was increased, but hAMSCs transplantation reversed this effect. Overal , hAMSCs transplantation can reduce neuronal apoptosis by reducing MAG and OMgp expression levels, and thereby promote neurological functional recovery from I/R injury in rats.关键词
干细胞/移植/人羊膜间充质干细胞/缺血再灌注损伤/神经功能/MAG/OMgp/大鼠分类
医药卫生引用本文复制引用
赵宇..尾静脉移植羊膜间充质干细胞对缺血再灌注损伤后神经功能恢复的影响[J].中国组织工程研究,2017,38(5):707-712,6.基金项目
天津市卫生局科技基金(2014KZ020)Funding:the Science and Technology Fund of Tianjin Health Department, No.2014KZ020 （2014KZ020）
