中国组织工程研究2017，Vol.38Issue(5)：713-717,5.DOI:10.3969/j.issn.2095-4344.2017.05.010
磁标记内皮祖细胞移植对糖尿病大鼠肾功能改善的作用：MRI成像验证
Effect of magnetic labeled endothelial progenitor cell transplantation on renal function of diabetic rats:a MRI imaging verification
摘要
Abstract
BACKGROUND:Endothelial progenitor cel s have been shown to play an important role in the pathogenesis of traumatic diseases in recent years. OBJECTIVE:To explore the effect of magnetic labeled endothelial progenitor cel transplantation on renal function of diabetic rats through a MRI imaging study.METHODS:Sixty Wistar rats were randomly divided into normal (no treatment), control and experimental groups. Intraperitoneal injection of 40 mg/kg streptozotocin was performed to make a rat model of type 1 diabetes in the control and experimental groups. Four weeks after modeling, rats in the experimental group were given intravenous injection of magnetic labeled endothelial progenitor cel s (0.15 mL, 1×109/L). Fasting blood glucose, serum insulin, serum creatinine, urea nitrogen and 24-hour urinary protein levels in rats were measured at 8 weeks after cel transplantation. MRI was used to trace transplanted cel s in vivo in comparison with renal biopsy findings, and rat body mass and kidney weight were measured to calculate kidney weight index. RESULTS AND CONCLUSION:After modeling, fasting blood glucose, serum creatinine, urea nitrogen and 24-hour urinary protein levels as wel as kidney weight index were increased significantly (P<0.05), while the insulin level decreased (P<0.05). Compared with the model group, the endothelial progenitor cel transplantation reversed these indices (P<0.05). Additional y, in the experimental group, there was slightly longer T1 and shorter T2 signals as wel as marked lesion edge, and the FLASH sequence became more remarkable compared with the T2-weighted RARE sequence. The other groups showed no significant low signal changes. Magnetic-labeled positive cel s in the experimental group showed by the MRI were consistent with the tissue biopsy results, while no positive cel s were found in the model and normal groups. To conclude, the magnetic labeled endothelial progenitor cel transplantation can improve renal dysfunction in diabetic rats to a certain extent.关键词
干细胞/移植/磁标记/内皮祖细胞移植/大鼠/糖尿病/肾功能/MRI成像/国家自然科学基金分类
医药卫生引用本文复制引用
冯娜,徐英进,董曦,杨晶菁,何新..磁标记内皮祖细胞移植对糖尿病大鼠肾功能改善的作用：MRI成像验证[J].中国组织工程研究,2017,38(5):713-717,5.基金项目
国家自然科学基金面上项目(51473044)Funding:the National Natural Science Foundation of China, No.51473044 （51473044）
