中国组织工程研究2017，Vol.38Issue(5)：730-735,6.DOI:10.3969/j.issn.2095-4344.2017.05.013
补肾活血方联用神经干细胞移植治疗大鼠耳鸣
Effects of Bushen Huoxue Recipe combined with neural stem cell transplantation in rats with tinnitus
摘要
Abstract
BACKGROUND:Neural stem cel s have multi-directional differentiation potential, self-sustaining and self-renewal capacity as wel as have strong migration ability. Bushen Huoxue Recipe can reduce neuronal damage and promote nerve cel regeneration, to achieve neural function reconstruction. Underlying mechanisms of Bushen Huoxue Recipe combined with neural stem cel transplantation in rats with tinnitus induced by sodium salicylate are yet unclear. OBJECTIVE:To investigate the effects of Bushen Huoxue Recipe combined with neural stem cel transplantation in rats with tinnitus induced by sodium salicylate. METHODS:Sixty Sprague-Dawley rats were randomized into five groups (n=12):normal control group, tinnitus model group, Bushen Huoxue Recipe group, stem cel group and combined treatment group (Bushen Huoxue Recipe combined with neural stem cel transplantation). Animal models of tinnitus induced by sodium salicylate were made in al the groups except for the normal control group. Fifteen days after modeling, rats were given intragastric administration of Bushen Huoxue Recipe water decoction (3 mL, 2.592 g/mL) for consecutive 7 days in the Bushen Huoxue Recipe group, intravenous injection of neural stem cel s (1 mL, 1.0×109/L) in the stem cel group, or their combined treatment in the combined treatment group. RESULTS AND CONCLUSION:Bushen Huoxue Recipe, neural stem cel transplantation and their combination al could effectively promote the recovery of drinking water inhibitory rate that was ranked as fol ows:combined treatment group<Bushen Huoxue Recipe group<stem cel group. Additional y, the above-mentioned methods could effectively reduce pathological damage of primary auditory cortex in the rat brain. Western blot results showed higher 5-serotonin levels in the Bushen Huoxue Recipe group and combined treatment group than in the stem cel group, which were identical to ELISA findings. Our experimental findings indicate that Bushen Huoxue Recipe combined with neural stem cel transplantation exhibit certain effects on tinnitus induced by sodium salicylate in rats, and the mechanism of action is associated with neuronal repair and expression of 5-serotonin at mRNA and protein levels.关键词
干细胞/移植/补肾活血方/神经干细胞/耳鸣/5-羟色胺/Rb/western-blot/ELISA/病理形态分类
医药卫生引用本文复制引用
王荣国,皮丽宏,陈红耀,张海中..补肾活血方联用神经干细胞移植治疗大鼠耳鸣[J].中国组织工程研究,2017,38(5):730-735,6.基金项目
2014年度河北省医学科学研究重点课题计划指令性课题(ZL20140084)Funding:the Hebei Provinical Medical Science Research Project in 2014, No. ZL20140084 （ZL20140084）
