中国组织工程研究2017，Vol.38Issue(5)：755-759,5.DOI:10.3969/j.issn.2095-4344.2017.05.017
人胎盘来源间充质干细胞的生物学性能及体外生物相容性
Biological characterization and in vitro biocompatibility of human placenta derived mesenchymal stem cells
摘要
Abstract
BACKGROUND:At present bone marrow mesenchymal stem cel s act as the main seed cel s in bone tissue engineering, but only 0.001%-0.01%cel s are in the bone with difficulty in cel separation and purification. OBJECTIVE:To explore the biological characterization of human placenta derived mesenchymal stem cel s and biocompatibility with three-dimensional porous hydroxyapatite ceramic scaffold. METHODS:Human placenta derived mesenchymal stem cel s were morphological y observed and identified usingflow cytometry, fol owed by osteogenic, adipogenic, chondrogenic induction for 3 weeks. Afterwards, the potential of multi-directional differentiation was identified by alizarin red S, oil red O and toluidine blue staining. DAPI staining was used to observe the adhesion of cel s on the surface of the hydroxyapatite ceramic scaffold under scanning electron microscope. RESULTS AND CONCLUSION:The human placenta derived mesenchymal stem cel s showed long spindle shape and uniform size under the microscope;they highly expressed CD29 and D90, but did not express CD45 and CD106. Fol owing induction, mineralized nodules were observed by alizarin red S staining, lipid droplets by oil red O staining and blue-dyed toluidine blue staining. These cel s adhered wel to the scaffold surface, indicting they are suitable for bone tissue engineering.关键词
干细胞/分化/胎盘间充质干细胞/流式细胞仪/多向分化潜能/羟基磷灰石陶瓷支架/生物相容性分类
医药卫生引用本文复制引用
伍佳,温永梅,吕欣荣,牟雁东..人胎盘来源间充质干细胞的生物学性能及体外生物相容性[J].中国组织工程研究,2017,38(5):755-759,5.基金项目
四川省科技厅项目(2016TD0008，2014JY0187)Funding:the Projects of Sichuan Provincial Science and Technology Department, No.2016TD0008,2014JY0187 （2016TD0008，2014JY0187）
