中国组织工程研究2017，Vol.38Issue(5)：760-765,6.DOI:10.3969/j.issn.2095-4344.2017.05.018
子宫内膜基质干细胞的体外分离培养及生物学特性
Endometrial stromal stem cells:in vitro isolation, culture and biological features
毕玉虎 1滕君儒1
作者信息
- 1. 济南市第三人民医院，山东省济南市 250132
- 折叠
摘要
Abstract
BACKGROUND:Understanding and studying endometrial cel s from the perspective of stem cel s can provide a new therapeutic approach and research entry point for the clinical treatment of endometrial diseases. OBJECTIVE:To compare the biological features of endometrial stromal stem cel s isolated and cultured using different methods. METHODS:Three commonly used cel separation methods, including trypsin, col agenase I and their combination, were used to isolate and culture endometrial stromal stem cel s from the uterus after removal. Afterwards, passage 2 cel s cultured for 5 days were subjected to trypan blue staining and living cel counting. Immunohistochemical staining for CD146 and CD90 and RT-PCR were used to identify harvested endometrial stromal stem cel s. Logarithmical y growing cel s were cultured to draw cel growth curves. RESULTS AND CONCLUSION:Harvested endometrial stromal stem cel s presented with spindle, adherent cel growth, nonpolar arrangement, and fibroblast morphology. The DNA of endometrial stem cel marker CD146 andCD90 highly expressed in 500 bp and 150 bp respectively, but did not express in the mesometrium. S-shaped growth curve of cultured cel s was found, and there was no difference in the cel cycle of cel s cultured using three different methods. Among the three methods, col agenase I method could harvest the highest number of endometrial stromal stem cel s that grew fastest. These findings indicate that endometrial stromal stem cel s can be successful y isolated from the removed uterine tissues, and col agenase I method is an efficient, practical and stable method for in vitro culture of endometrial stromal stem cel s.关键词
干细胞/分化/子宫/内膜基质/细胞培养/细胞分化分类
医药卫生引用本文复制引用
毕玉虎,滕君儒..子宫内膜基质干细胞的体外分离培养及生物学特性[J].中国组织工程研究,2017,38(5):760-765,6.
