中国组织工程研究2017，Vol.38Issue(5)：773-779,7.DOI:10.3969/j.issn.2095-4344.2017.05.020
人胎盘胎儿侧间充质干细胞无血清培养上清的抗氧化活性分析
Anti-oxidative activities of supernatants of human fetal placental mesenchymal stem cells cultured in serum-free medium
摘要
Abstract
BACKGROUND:Current research on mesenchymal stem cels (MSCs) is mostly focused on its immune regulatory function, while little is reported on the antioxidant capacity of the cels and culture supernatant.OBJECTIVE: To investigate the anti-oxidative capacity of the supernatant harvested from human fetal placenta MSCs (fPMSCs) under a condition of serum free culture. METHODS:fPMSCs were cultured with serum free media, and the supernatants of cels at passages 2-6 were colected at 48 hours after culture. Vitamin C was added into the culture medium, as a positive control, and its concentration was 100 μmol/L. The total antioxidant capacity, scavenging capacity of free radicals and antioxidant enzymatic activities of supernatants were measured. RESULTS AND CONCLUSION: By comparing anti-oxidative activities of vitamin C and na?ve culture medium, supernatants colected from fPMSCs cultures exhibited obvious antioxidant capacities at different extents between passages of cel cultures. The total antioxidant capacity of the culture supernatant was comparable to 40-80 μmol/L vitamin C. In addition, al supernatants derived from cels with different passages displayed capacities to scavenge free radicals, including 2,2-diphenyl-1-picrylhydrazyl radical (DPPH?), hydroxyl radical (?OH), superoxide anion radical (O2-). Even more, activities of antioxidant enzymes, including superoxide dismutase and glutathione peroxidase, were also detected in supernatants colected from different passages of fPMSCs. Under the serum-free condition, the culture supernatants of fPMSCs have antioxidant capacities at certain extent. However, the antioxidant components and underlying mechanisms need to be further studied.关键词
干细胞/分化/胎盘/胎儿侧/间充质干细胞/无血清培养/培养上清/抗氧化/自由基清除/抗氧化酶/旁分泌/无细胞治疗/国家自然科学基金分类
医药卫生引用本文复制引用
付雪,张玉洁,严秀蕊,马晓娜,刘晓明,魏军..人胎盘胎儿侧间充质干细胞无血清培养上清的抗氧化活性分析[J].中国组织工程研究,2017,38(5):773-779,7.基金项目
国家自然科学基金(81460247)Funding:the National Natural Science Foundation of China, No.81460247 （81460247）
